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Western Blot (WB) (Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.)

Rabbit anti-Human, Mouse UBAP2L Polyclonal Antibody | anti-UBAP2L antibody

UBAP2L Antibody

Gene Names
UBAP2L; NICE4; NICE-4
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
Affinity chromatography purified via peptide column.
Synonyms
UBAP2L; Polyclonal Antibody; UBAP2L Antibody; Ubiquitin associated protein 2-like; NICE-4; anti-UBAP2L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist; this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1087
Applicable Applications for anti-UBAP2L antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry at 10 mug/ml.
Immunogen
UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
HeLa Cell Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.)

Western Blot (WB) (Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.)

Immunocytochemistry (IHC) (Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.Red: UBAP2L Antibody (8371)Blue: DAPI staining)

Immunofluorescence (IF) (Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.Red: UBAP2L Antibody (8371)Blue: DAPI staining)
Related Product Information for anti-UBAP2L antibody
The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).
Product Categories/Family for anti-UBAP2L antibody
References
Bordeleau ME, Aucagne R, Chagraoui J, et al. UBAPL2L is a novel BMI1-iteracting protein essential for hematopoietic stem cell activity. Blood 2014; 124:2362-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 120 kDa
Observed: 130 kDa
NCBI Official Full Name
ubiquitin-associated protein 2-like isoform a
NCBI Official Synonym Full Names
ubiquitin associated protein 2 like
NCBI Official Symbol
UBAP2L
NCBI Official Synonym Symbols
NICE4; NICE-4
NCBI Protein Information
ubiquitin-associated protein 2-like
UniProt Protein Name
Ubiquitin-associated protein 2-like
UniProt Gene Name
UBAP2L
UniProt Synonym Gene Names
KIAA0144; NICE4
UniProt Entry Name
UBP2L_HUMAN

Uniprot Description

NICE-4: a ubiquitous protein containing an UBA-domain, which are present in multiple proteins of the ubiquitination pathway. Encoded by a gene of the human epidermal differentiation complex (EDC). May be involved in epidermal differentiation. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; RNA-binding

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: PcG protein complex

Molecular Function: protein binding

Biological Process: binding of sperm to zona pellucida

Research Articles on UBAP2L

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Product Notes

The UBAP2L ubap2l (Catalog #AAA154382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBAP2L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBAP2L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry at 10 mug/ml. Researchers should empirically determine the suitability of the UBAP2L ubap2l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBAP2L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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