Mouse anti-Human. Estrogen Receptor Monoclonal Antibody | anti-ESR1 antibody

Estrogen Receptor

Cat. Num. AAA438468

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human.
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: Estrogen Receptor; Monoclonal Antibody; Estrogen Receptor alpha delta 4*5;6;7*/654 isoform; Estrogen Receptor alpha delta 4 +49 isoform; Nuclear receptor subfamily 3 group A member 1; anti-ESR1 antibody

• Host: Mouse
• Reactivity: Human.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ER506
• Specificity: This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 595

• Applicable Applications for anti-ESR1 antibody: Flow Cytometry (FC/FACS), Immunohistology (IH), Western Blot (WB)
• Application Notes: Flow Cytometry (0.5-1ug/million cells) Immunohistology (Frozen & Formalin-paraffin) Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Western Blotting (0.5-1ug/ml for 2 hours at Room Temp) Optimal dilution for a specific application should be determined.
• Localization: Nuclear
• Positive Control: MCF-7 Cells or Breast Cancers.
• Human Chromosomal Location: 6q25.1
• Immunogen: Recombinant human Estrogen Receptor alpha protein (aa2-185)
• Supplied as: 200ug/ml of Ab purified fro Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available Without BSA & azdie at 1.0mg/ml.
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months.

Flow Cytometry

(Flow Cytometry for human ER-alpha on MCF-7 Cells.Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-alpha Monoclonal Antibody (ER506).)

Formalin-paraffin

(Formalin-paraffin human breast invasive ductal carcinoma stained with Estrogen Receptor MAb (ER506))

References

1. Zafrani B, et. al. Histopathology 2000; 37(6), 536-545.
2. Harvey JM, et. al. Journal of Clinical Oncology 1999; 17(5), 1474-1481.

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: ~67kDa
• NCBI Official Full Name: estrogen receptor isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; negative regulation of transcription factor activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance