Human Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1) ELISA Kit | RAP1GDS1 elisa kit

Human Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1) ELISA Kit

Cat. Num. AAA269981

• Gene Names: RAP1GDS1; GDS1; SmgGDS
• Reactivity: Human
• Synonyms: Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1); Human Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1) ELISA Kit; RAP1GDS1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 608

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PAP1GDS1 concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAP1GDS1 elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PAP1GDS1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 155030192
• NCBI GeneID: 5910
• NCBI Accession #: NP_001093896.1
• NCBI GenBank Nucleotide #: NM_001100426.1
• Molecular Weight: 56,536 Da
• NCBI Official Full Name: rap1 GTPase-GDP dissociation stimulator 1 isoform 1
• NCBI Official Synonym Full Names: RAP1, GTP-GDP dissociation stimulator 1
• NCBI Official Symbol: RAP1GDS1
• NCBI Official Synonym Symbols: GDS1; SmgGDS
• NCBI Protein Information: rap1 GTPase-GDP dissociation stimulator 1; SMG GDS protein; exchange factor smgGDS; SMG P21 stimulatory GDP/GTP exchange protein
• UniProt Protein Name: Rap1 GTPase-GDP dissociation stimulator 1
• Protein Family: Rap1 GTPase-GDP dissociation stimulator
• UniProt Gene Name: RAP1GDS1
• UniProt Entry Name: GDS1_HUMAN

NCBI Description

The smg GDP dissociation stimulator (smgGDS) protein is a stimulatory GDP/GTP exchange protein with GTPase activity (Riess et al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010]

Uniprot Description

RAP1GDS1: Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Chromosomal Location of Human Ortholog: 4q23-q25

Cellular Component: mitochondrion; endoplasmic reticulum; cytosol

Molecular Function: GTPase activator activity

Biological Process: reduction of endoplasmic reticulum calcium ion concentration; vascular smooth muscle contraction; myosin filament assembly; elevation of mitochondrial calcium ion concentration