Mouse ESET Monoclonal Antibody | anti-ESET antibody

ESET Mouse mAb

Cat. Num. AAA8538709

• Gene Names: SETDB1; ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• Reactivity: Human, Mouse, Monkey
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Ascitic Fluid
• Synonyms: ESET; Monoclonal Antibody; ESET Mouse mAb; KG1T; KMT1E; KIAA0067; H3-K9-HMTase4; SETDB1; anti-ESET antibody

• Host: Mouse
• Reactivity: Human, Mouse, Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1E7-F3-C6
• Purity/Purification: Ascitic Fluid
• Form/Format: Ascitic fluid containing 0.03% Sodium Azide.
• Sequence Length: 1291

• Applicable Applications for anti-ESET antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ICC: 1:200-1:1000; ELISA: 1:10000
• Immunogen: Purified recombinant fragment of human SETDB1 expressed in E. Coli.
• Cellular Localization: Chromosome, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Related Product Information for anti-ESET antibody

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 25091210
• NCBI GeneID: 9869
• UniProt Accession #: Q15047
• Molecular Weight: 180kDa
• NCBI Official Full Name: Histone-lysine N-methyltransferase SETDB1
• NCBI Official Synonym Full Names: SET domain bifurcated histone lysine methyltransferase 1
• NCBI Official Symbol: SETDB1
• NCBI Official Synonym Symbols: ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
• NCBI Protein Information: histone-lysine N-methyltransferase SETDB1
• UniProt Protein Name: Histone-lysine N-methyltransferase SETDB1
• UniProt Gene Name: SETDB1
• UniProt Synonym Gene Names: KIAA0067; KMT1E; ESET; H3-K9-HMTase 4
• UniProt Entry Name: SETB1_HUMAN

NCBI Description

This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]

Uniprot Description

SETDB1: a protein lysine methyltransferase that specifically trimethylates K9 of histone H3 (H3K9me3), a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Unlike SUV39H H3K9 methyltransferase, which functions mainly in heterochromatin regions such as pericentric heterochromatin, SETDB1 functions mainly in euchromatic regions, playing a central role in the silencing of euchromatic genes. H3K9me3 is coordinated with DNA methylation. Interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. Contains Tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. Is targeted to histone H3 by TIF1B, a factor recruited by KRAB zinc-finger proteins. Recruited by DNMT3A to silenced promoters in cancer cells. May play a role in the pathogenesis of Huntington's disease, since levels of SETDB1 and H3K9me3 are both increased in diseased brains. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation