Rabbit anti-Human MTAP Polyclonal Antibody | anti-MTAP antibody

MTAP, NT (MTAP, MSAP, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase) (FITC)

Cat. Num. AAA6322684

• Gene Names: MTAP; BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MTAP; Polyclonal Antibody; NT (MTAP; MSAP; S-methyl-5'-thioadenosine phosphorylase; 5'-methylthioadenosine phosphorylase) (FITC); 5'-methylthioadenosine phosphorylase; anti-MTAP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MTAP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human MTAP.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MTAP Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the MTAP antibody detected the MTAP protein (arrow).)

Related Product Information for anti-MTAP antibody

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Product Categories/Family for anti-MTAP antibody

Antibodies; Enzymes; Phosphorylase

References

Newton-Bishop, J.A., et al. Cancer Epidemiol. Biomarkers Prev. 19(8):2043-2054(2010). Yang, X.R., et al. Fam. Cancer (2010). In press: Krasinskas, A.M., et al. Mod. Pathol. 23(4):531-538(2010). Zhang, Q., et al. Zhonghua Liu Xing Bing Xue Za Zhi 31(1):83-86(2010). Huang, H.Y., et al. Clin. Cancer Res. 15(22):6963-6972(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 47132622
• NCBI GeneID: 4507
• NCBI Accession #: NP_002442.2
• NCBI GenBank Nucleotide #: NM_002451.3
• UniProt Accession #: Q13126; Q5T3P3; Q9H010; I2G7M5; I2G7M6; I2G7M7; I2G7M8; I2G7M9; I2G7N0
• Molecular Weight: 26,278 Da
• NCBI Official Full Name: S-methyl-5'-thioadenosine phosphorylase
• NCBI Official Synonym Full Names: methylthioadenosine phosphorylase
• NCBI Official Symbol: MTAP
• NCBI Official Synonym Symbols: BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249
• NCBI Protein Information: S-methyl-5'-thioadenosine phosphorylase
• UniProt Protein Name: S-methyl-5'-thioadenosine phosphorylase
• UniProt Gene Name: MTAP
• UniProt Entry Name: MTAP_HUMAN

NCBI Description

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]