Testing Data
Rat anti-Mouse Erythroid Cell (Ly 76) Monoclonal Antibody
Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b)
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
Erythroid Cell (Ly 76); Monoclonal Antibody; Anti-Mouse Erythroid Cell (Ly 76); Biotin (Clone TER119) (rat IgG2b); Biotin Anti-Mouse Erythroid Cell (Ly-76) Monoclonal Antibody; anti-Erythroid Cell (Ly 76) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2b
Clone Number
TER-119
Specificity
Erythroid Cells (Ly-76) (Mouse Erythroid Cells (Ly-76))
Form/Format
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from supernatant via Protein G Chromatography).)
Concentration
Antibody Concentration: 0.1 mg/ml (varies by lot)
Applicable Applications for anti-Erythroid Cell (Ly 76) antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Immunogen
Day 14 BALB/c fetal liver cells
Donor
Wistar rat spleen
Fusion Partner
X63.Ag8.653 myloma cells
Presentation
100 ug or 300ug Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
Preparation and Storage
Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-Erythroid Cell (Ly 76) antibody
anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER119) is specific for cells at stages from early proerythroblast to mature erythrocytes. TER119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive. TER119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER119 in Western blot analysis. It has been determined that the TER119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells. This antibody is suitable for use in flow cytometry. It has also be reported to work in Western Blotting, immunoprecipitation and immunohistochemistry (frozen and paraffin).
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Product Notes
The Erythroid Cell (Ly 76) (Catalog #AAA520283) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Erythroid Cell (Ly 76), Biotin (Clone TER119) (rat IgG2b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Erythroid Cell (Ly 76) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Erythroid Cell (Ly 76) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Erythroid Cell (Ly 76), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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