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Western Blot (WB) (Western Blot analysis of K-562 cells using GTF2F1 Polyclonal Antibody at dilution of 1:1000)

Rabbit GTF2F1 Polyclonal Antibody | anti-GTF2F1 antibody

GTF2F1 Antibody

Gene Names
GTF2F1; BTF4; RAP74; TF2F1; TFIIF
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
GTF2F1; Polyclonal Antibody; GTF2F1 Antibody; BTF4; RAP74; TF2F1; TFIIF; TFIIF alpha; anti-GTF2F1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
517
Applicable Applications for anti-GTF2F1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
fusion protein of GTF2F1
Note
Centrifuge before opening to ensure complete recovery of vial contents.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-235 / sc-234
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of K-562 cells using GTF2F1 Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of K-562 cells using GTF2F1 Polyclonal Antibody at dilution of 1:1000)
Related Product Information for anti-GTF2F1 antibody
In eukaryotic systems, the initiation of gene transcription involves the ordered assembly of a multiprotein complex on proximal promoter elements, consisting of RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, B, D, E, F and H; and sequence specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. TFIIF, a heteromer composed of a small (RAP 30) and a large (RAP 74) subunit, acting at an intermediate stage in initiation complex formation, binds directly to RNA polymerase II in solution and decrease the affinity of RNA polymerase II for nonspecific DNA. In addition, TFIIF stimulates transcription elongation by RNA polymerase II.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated MW: 58 kDa
Observed MW: 74 kDa
NCBI Official Full Name
GTF2F1
NCBI Official Synonym Full Names
general transcription factor IIF, polypeptide 1, 74kDa
NCBI Official Symbol
GTF2F1
NCBI Official Synonym Symbols
BTF4; RAP74; TF2F1; TFIIF
NCBI Protein Information
general transcription factor IIF subunit 1; TFIIF-alpha; general transcription factor IIF 74 kDa subunit; transcription initiation factor IIF subunit alpha; transcription initiation factor RAP74
UniProt Protein Name
GTF2F1 protein
UniProt Gene Name
GTF2F1
UniProt Entry Name
Q6IBK5_HUMAN

Uniprot Description

GTF2F1: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2, CTDP1, TAF6/TAFII80 and URI1. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the TFIIF alpha subunit family.

Protein type: Kinase, protein; Transcription initiation complex; EC 2.7.11.1; ATYPICAL group; GTF2F1 family

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; transcription factor TFIIF complex; cell junction; nucleus

Molecular Function: protein binding; DNA binding; phosphatase activator activity; transcription coactivator activity; transcription factor binding; catalytic activity

Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; positive regulation of catalytic activity; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; positive regulation of viral transcription; positive regulation of RNA elongation from RNA polymerase II promoter; RNA splicing; response to virus; RNA elongation from RNA polymerase II promoter; gene expression

Research Articles on GTF2F1

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Product Notes

The GTF2F1 gtf2f1 (Catalog #AAA2528453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF2F1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2F1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the GTF2F1 gtf2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2F1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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