Immunohistochemistry (IHC)
(erbB-4/HER-4 staining in aggressive ER+ in situ breast carcinoma (left image) and in pancreatic cancer (right image), tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB. Note: In the indolent cancer forms (for breast) weak cytoplasmic staining was observed without the focal strong plasma membrane staining which is observed in the aggressive form of ductal carcinoma.)
Mouse erbB4 Monoclonal Antibody | anti-erbB4 antibody
erbB4 (c-erbB4, HER4) (MaxLight 550)
Immunohistochemistry (IHC)
(erbB-4/HER-4 staining in aggressive ER+ in situ breast carcinoma (left image) and in pancreatic cancer (right image), tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB. Note: In the indolent cancer forms (for breast) weak cytoplasmic staining was observed without the focal strong plasma membrane staining which is observed in the aggressive form of ductal carcinoma.)
Immunohistochemistry (IHC)
(erbB-4/HER-4 staining in aggressive ER+ in situ breast carcinoma (left image) and in pancreatic cancer (right image), tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select® detection with HRP-DAB. Note: In the indolent cancer forms (for breast) weak cytoplasmic staining was observed without the focal strong plasma membrane staining which is observed in the aggressive form of ductal carcinoma.)
Western Blot (WB)
(Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-C-ERB-4(1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemilumin scence detection system. Arrow indicates protein C-ERB-4 (~147kD).)
Western Blot (WB)
(Western Blot Analysis: A431 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-C-ERB-4(1:500 dilution of this lot). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemilumin scence detection system. Arrow indicates protein C-ERB-4 (~147kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.
Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; EGFR family
Chromosomal Location of Human Ortholog: 2q33.3-q34
Cellular Component: nucleoplasm; mitochondrion; basolateral plasma membrane; mitochondrial matrix; plasma membrane; extracellular region; integral to membrane; cytosol; nucleus; receptor complex
Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding
Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; heart development; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; embryonic pattern specification; signal transduction; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; cell fate commitment; phosphoinositide-mediated signaling; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of protein amino acid phosphorylation; mitochondrial fragmentation during apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis
Disease: Amyotrophic Lateral Sclerosis 19
Research Articles on erbB4
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Product Notes
The erbB4 erbb4 (Catalog #AAA6246181) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The erbB4 (c-erbB4, HER4) (MaxLight 550) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's erbB4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the erbB4 erbb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "erbB4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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