Mouse ErbB3 Monoclonal Antibody | anti-ErbB3 antibody

MOUSE ANTI HUMAN ErbB3

Cat. Num. AAA212132

• Gene Names: ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: ErbB3; Monoclonal Antibody; MOUSE ANTI HUMAN ErbB3; anti-ErbB3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SGP1
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 183

• Applicable Applications for anti-ErbB3 antibody: Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunoprecipitation (IP)
• Application Notes: Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-ErbB3 antibody

Mouse anti Human ErbB3 antibody, clone SGP1 recognizes human Receptor tyrosine-protein kinase erbB-3, also known as Proto-oncogene-like protein c-ErbB-3 or Tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid ~180 kDa glycoprotein containing a single protein kinase domain. Five isoforms of Erb-B3 are generated by alternative splicing. Mouse anti Human ErbB3 antibody, clone SGP1 recognizes a conformation dependent epitope on the extracellular protein core of ErbB3 rather than a carbohydrate moiety created during post-translational modification. ErbB3 is expressed widely in normal human tissues, but is over expressed in some tumours, including those of the breast, stomach, pancreas and colon (Lemoine et al. 1992). Mutations in the ERBB3 gene can lead to development of Lethal congenital contractural syndrome type 2, associated with atrophy of the spinal cord (Narkis et al. 2007).

NCBI and Uniprot Product Information

• NCBI GI #: 54792102
• NCBI GeneID: 2065
• NCBI Accession #: NP_001005915.1
• NCBI GenBank Nucleotide #: NM_001005915.1
• UniProt Accession #: P21860; Q9BUD7; A8K6L6; B4DIK7; B4DV32; E9PDT8
• Molecular Weight: 77,426 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-3 isoform s
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 3
• NCBI Official Symbol: ERBB3
• NCBI Official Synonym Symbols: HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-3; human epidermal growth factor receptor 3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-3
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB3
• UniProt Synonym Gene Names: HER3
• UniProt Entry Name: ERBB3_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER3: a receptor tyrosine kinase of the EGFR family. Binds and is activated by neuregulins and NTAK. Can form homodimers or ErbB-2/ErbB-3 heterodimers. Kinase domain lacks activity but heterodimerizes with other EGFRs to transduce growth signals. May be required for HER2 activity. Elevated expression in breast and other tumors is indicative of poor outcome. A secreted form is expressed in metastatic prostate cancer Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, tyrosine (receptor); Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Membrane protein, integral; Kinase, protein; TK group; EGFR family

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; lateral plasma membrane; receptor complex

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; protein homodimerization activity; protein heterodimerization activity; growth factor binding; protein-tyrosine kinase activity; protein tyrosine kinase activator activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide 3-kinase cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; wound healing; nerve growth factor receptor signaling pathway; heart development; cranial nerve development; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; regulation of cell proliferation; peripheral nervous system development; negative regulation of signal transduction; positive regulation of phosphoinositide 3-kinase cascade; Schwann cell differentiation; neuron apoptosis; innate immune response; negative regulation of secretion; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lethal Congenital Contracture Syndrome 2