Western Blot (WB)
(Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1, 000 dilution.)
Mouse anti-Human EPN1 Monoclonal Antibody | anti-EPN1 antibody
EPN1 Antibody
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
EPN1; Monoclonal Antibody; EPN1 Antibody; EH domain binding mitotic phosphoprotein antibody; EH domain-binding mitotic phosphoprotein antibody; EPN 1 antibody; Epn1 antibody; EPN1_HUMAN antibody; EPS 15 interacting protein 1 antibody; EPS-15-interacting protein 1 antibody; Epsin 1 antibody; Epsin-1 antibody; anti-EPN1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
F9-F7
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-EPN1 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
FC/FACS: 1:50-1:100
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1, 000 dilution.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-EPN1 antibody
Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the alpha subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent.
Product Categories/Family for anti-EPN1 antibody
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types. [provided by RefSeq, Mar 2016]
Uniprot Description
Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (). Regulates receptor-mediated endocytosis.
Research Articles on EPN1
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Product Notes
The EPN1 epn1 (Catalog #AAA9435249) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EPN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EPN1 epn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPN1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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