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ZIKV (strain Zika SPH2015) Envelope protein Recombinant Protein | ZIKV recombinant protein
Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, Fc Tag)
Purity
>90% as determined by SDS-PAGE
Synonyms
ZIKV (strain Zika SPH2015) Envelope protein; Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III; Fc Tag); Zika virus; E protein; strain Zika SPH2015; ZIKV recombinant protein
Host
HEK293 Cells
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Sequence
Val593-Lys699
Species
Zika virus (ZIKV)
Tag
C-hFc1
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Protein Construction
A DNA sequence encoding the Zika virus (strain Zika SPH2015) E/Envelope (domain III)(ALU33341.1)(Val593-Lys699) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
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Related Product Information for ZIKV recombinant protein
Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
Product Categories/Family for ZIKV recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Mol. Mass: 38.2kDa
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Product Notes
The ZIKV (Catalog #AAA2569600) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Val593-Lys 699. It is sometimes possible for the material contained within the vial of "ZIKV (strain Zika SPH2015) Envelope protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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