Hamster anti-Human Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 3 Monoclonal Antibody | anti-EMR2 antibody

Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 3 (EGF-like Module Containing Mucin-like Hormone Receptor-like 3, EGF-like Module Containing Mucin-like Receptor 3, EMR3)

Cat. Num. AAA605832

• Gene Names: EMR2; CD312
• Reactivity: Human
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 3; Monoclonal Antibody; Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 3 (EGF-like Module Containing Mucin-like Hormone Receptor-like 3; EGF-like Module Containing Mucin-like Receptor 3; EMR3); Anti -Epidermal Growth Factor-Like Module-Containing Mucin-Like Receptor 3 (EGF-like Module Containing Mucin-like Hormone Receptor-like 3; anti-EMR2 antibody

• Host: Hamster
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10C111
• Specificity: Recognizes human epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-EMR2 antibody: Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry and Immunoprecipitation.; Dilution: Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul
• Immunogen: ARHO-EMR3-CD97 (EGF1) transfectants.
• Hybridoma: SP2/0 myeloma cells with spleen cells from Armenian hamster.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-EMR2 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 108935835
• NCBI GeneID: 30817
• UniProt Accession #: Q9UHX3; Q8NG96; Q9Y4B1; B4DQ96; E7ESD7; E9PBR1; E9PEL6; E9PFQ5; E9PG91
• Molecular Weight: 90,472 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 2
• NCBI Official Synonym Full Names: egf-like module containing, mucin-like, hormone receptor-like 2
• NCBI Official Symbol: EMR2
• NCBI Official Synonym Symbols: CD312
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 2
• UniProt Gene Name: EMR2
• UniProt Entry Name: EMR2_HUMAN

NCBI Description

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

EMR2: Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 2; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell migration; cell adhesion; inflammatory response