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Western Blot (WB) (Western Blot detection against Immunogen (65.23kD).)

Mouse anti-Human Decorin Monoclonal Antibody | anti-DCN antibody

Decorin (DCN, Bone Proteoglycan II, CSCD, DSPG2, PG40, PGII, PGS2, Small Leucine Rich Protein 1B, SLRR1B) (AP)

Gene Names
DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Decorin; Monoclonal Antibody; Decorin (DCN; Bone Proteoglycan II; CSCD; DSPG2; PG40; PGII; PGS2; Small Leucine Rich Protein 1B; SLRR1B) (AP); anti-DCN antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3H4-1F4
Specificity
Recognizes human DCN.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-DCN antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-359 from human DCN (AAH05322) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (65.23kD).)

Western Blot (WB) (Western Blot detection against Immunogen (65.23kD).)

Western Blot (WB)

(Western Blot analysis of DCN expression in transfected 293T cell line by DCN monoclonal antibody Lane 1: DCN transfected lysate (39.7kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of DCN expression in transfected 293T cell line by DCN monoclonal antibody Lane 1: DCN transfected lysate (39.7kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DCN on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to DCN on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged DCN is ~1ng/ml as a capture antibody)

Testing Data (Detection limit for recombinant GST tagged DCN is ~1ng/ml as a capture antibody)
Product Categories/Family for anti-DCN antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
8,266 Da
NCBI Official Full Name
Homo sapiens decorin, mRNA
NCBI Official Synonym Full Names
decorin
NCBI Official Symbol
DCN
NCBI Official Synonym Symbols
CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
NCBI Protein Information
decorin
Protein Family

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]

Research Articles on DCN

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Product Notes

The DCN (Catalog #AAA6130903) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Decorin (DCN, Bone Proteoglycan II, CSCD, DSPG2, PG40, PGII, PGS2, Small Leucine Rich Protein 1B, SLRR1B) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Decorin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DCN for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Decorin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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