Western Blot (WB)
(The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : EPHX1 transfected 293T cell lysate)
Mouse anti-Human, Mouse EPHX1 Monoclonal Antibody | anti-EPHX1 antibody
EPHX1 antibody
Recommended starting dilution is 1:1000.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : EPHX1 transfected 293T cell lysate)
Western Blot (WB)
(The extracts of mouse liver (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPHX1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHX1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : EPHX1 transfected 293T cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of EPHX1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EPHX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of EPHX1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EPHX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2008]
Uniprot Description
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (). May play a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed:22798687).
Research Articles on EPHX1
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Product Notes
The EPHX1 ephx1 (Catalog #AAA200142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EPHX1 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EPHX1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ~ 2000. Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the EPHX1 ephx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHX1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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