Rabbit anti-Human p63 (Squamous, Basal & Myoepithelial Cell Marker) Monoclonal Antibody | anti-TP63 antibody

p63 (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody

Cat. Num. AAA4380164

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: p63 (Squamous; Basal & Myoepithelial Cell Marker); Monoclonal Antibody; Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody; Amplified in squamous cell carcinoma (AIS); Chronic ulcerative stomatitis protein (CUSP); EEC3; Keratinocyte transcription factor KET; LMS; NBP; p40; P51/P63; p53 like transcription factor; p53-related protein p63; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; TP63; TP73; TP73L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein 63; Tumor protein p53-like; tumor protein p73-like; anti-TP63 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.
• Form/Format: 200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-TP63 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Nuclear
• Immunogen: Recombinant human p63 protein fragment
• Positive Control: HEK293 cells or Prostate Carcinoma or Lung or bladder squamous cell carcinoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Cancer stained with p63 Rabbit Polyclonal Antibody.)

References

Signoretti S, et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol. 2000; 157(6):1769-75.
Terry J, et al. Optimal immunohistochemical markers for distinguishing lung adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg Pathol. 2010; 34(12):1805-11.

NCBI and Uniprot Product Information

• NCBI GeneID: 8626
• Molecular Weight: 63kDa
• Protein Family: Tumor protein

Uniprot Description

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.