Mouse anti-Human EPHB2 Monoclonal Antibody | anti-EPHB2 antibody

EPHB2 (Ephrin Type-B Receptor 2, Tyrosine-protein Kinase Receptor EPH-3, DRT, Receptor Protein-Tyrosine Kinase HEK5, ERK, EPH-like Kinase 5, EK5, Tyrosine-protein Kinase TYRO5, Renal Carcinoma Antigen NY-REN-47, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5) (MaxLi

Cat. Num. AAA6231055

• Gene Names: EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by protein G affinity chromatography followed by dialysis against PBS.
• Synonyms: EPHB2; Monoclonal Antibody; EPHB2 (Ephrin Type-B Receptor 2; Tyrosine-protein Kinase Receptor EPH-3; DRT; Receptor Protein-Tyrosine Kinase HEK5; ERK; EPH-like Kinase 5; EK5; Tyrosine-protein Kinase TYRO5; Renal Carcinoma Antigen NY-REN-47; EPHT3; EPTH3; HEK5; TYRO5) (MaxLi; anti-EPHB2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 10i37
• Specificity: Recognizes human EPHB2.
• Purity/Purification: Purified by protein G affinity chromatography followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-EPHB2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Purified His-tagged EPHB2 protein (fragment).
• Conjugate: MaxLight750
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EPHB2 recombinant protein (Fragment) using MBS632528 (1:2000). EPHB2 (Fragment) protein (arrow) was detected usingMBS632528.)

Western Blot (WB)

(Western Blot analysis of EPHB2 (arrow) usingMBS632528. Lane 1: Nontransfected 293 cell lysates (2ug/lane) Lane 2: transfected 293 cell lysates (2ug/lane) with the EphB2 (Origene Technologies).)

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma tissue usingMBS632528, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS632528 for immunohistochemistry.)

Testing Data

(Flow ytometric analysis of HepG2 cells using MBS632528 (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EPHB2 antibody

Antibodies; Tyrosine Kinases

References

1. Thanos, C.D., et al., Science 283(5403):833-836 (1999). 2.Tang, X.X., et al., Oncogene 17(4):521-526 (1998). 3.Fox, G.M., et al., Oncogene 10(5):897-905 (1995). 4.Ikegaki, N., et al., Hum. Mol. Genet. 4(11):2033-2045 (1995). 5.Iwase, T., et al., Biochem. Biophys. Res. Commun. 194(2):698-705 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 21396504
• NCBI GeneID: 2048
• NCBI Accession #: NP_004433.2
• NCBI GenBank Nucleotide #: NM_004442.6
• UniProt Accession #: P29323; O43477; Q5T0U6; Q5T0U7; Q5T0U8
• Molecular Weight: 117,493 Da
• NCBI Official Full Name: ephrin type-B receptor 2 isoform 2
• NCBI Official Synonym Full Names: EPH receptor B2
• NCBI Official Symbol: EPHB2
• NCBI Official Synonym Symbols: DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5
• NCBI Protein Information: ephrin type-B receptor 2; EPH-like kinase 5; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3; developmentally-regula
• UniProt Protein Name: Ephrin type-B receptor 2
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB2
• UniProt Synonym Gene Names: DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; EK5; hEK5
• UniProt Entry Name: EPHB2_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Membrane protein, integral; Tumor suppressor; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: cell soma; integral to plasma membrane; axon; dendrite; extracellular region; plasma membrane; cytosol

Molecular Function: protein binding; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; axon guidance receptor activity; ATP binding; receptor binding

Biological Process: nervous system development; axon guidance; inner ear morphogenesis; peptidyl-tyrosine phosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; learning; palate development; optic nerve morphogenesis; positive regulation of long-term neuronal synaptic plasticity; corpus callosum development; positive regulation of synaptogenesis; regulation of body fluid levels; camera-type eye morphogenesis; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; phosphorylation; urogenital system development; retinal ganglion cell axon guidance

Disease: Prostate Cancer/brain Cancer Susceptibility