Human Multimerin 1 ELISA Kit | MMRN1 elisa kit

Human Multimerin 1 ELISA Kit

Cat. Num. AAA764139

• Gene Names: MMRN1; ECM; MMRN; GPIa*; EMILIN4
• Reactivity: Human
• Synonyms: Multimerin 1; Human Multimerin 1 ELISA Kit; MMRN1/Multimerin 1; MMRN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MMRN1. No significant cross-reactivity or interference between MMRN1 and analogues was observed.
• Sequence Length: 1228

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMRN1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 39645323
• NCBI GeneID: 22915
• Molecular Weight: 58,170 Da
• NCBI Official Full Name: Multimerin 1
• NCBI Official Synonym Full Names: multimerin 1
• NCBI Official Symbol: MMRN1
• NCBI Official Synonym Symbols: ECM; MMRN; GPIa*; EMILIN4
• NCBI Protein Information: multimerin-1
• UniProt Protein Name: Multimerin-1
• Protein Family: Multimerin
• UniProt Gene Name: MMRN1
• UniProt Synonym Gene Names: ECM; EMILIN4; GPIA*; MMRN; Elastin microfibril interfacer 4; p-155; p155
• UniProt Entry Name: MMRN1_HUMAN

NCBI Description

Multimerin is a massive, soluble protein found in platelets and in the endothelium of blood vessels. It is comprised of subunits linked by interchain disulfide bonds to form large, variably sized homomultimers. Multimerin is a factor V/Va-binding protein and may function as a carrier protein for platelet factor V. It may also have functions as an extracellular matrix or adhesive protein. Recently, patients with an unusual autosomal-dominant bleeding disorder (factor V Quebec) were found to have a deficiency of platelet multimerin. [provided by RefSeq, Jul 2008]

Uniprot Description

MMRN1: Carrier protein for platelet (but not plasma) factor V/Va. Plays a role in the storage and stabilization of factor V in platelets. Upon release following platelet activation, may limit platelet and plasma factor Va-dependent thrombin generation. Ligand for integrin alpha-IIb/beta-3 and integrin alpha-V/beta-3 on activated platelets, and may function as an extracellular matrix or adhesive protein. Deficiency in multimerin-1 due to proteolytic degradation within the platelet alpha granules is associated with an autosomal dominant bleeding disorder (factor V Quebec). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: extracellular region

Biological Process: blood coagulation; cell adhesion; platelet activation; platelet degranulation