Mouse anti-Human Eosinophil Major Basic Protein Monoclonal Antibody | anti-PRG2 antibody

Eosinophil Major Basic Protein (Azide Free)

Cat. Num. AAA605121

• Gene Names: PRG2; MBP; BMPG; MBP1; MGC14537
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Eosinophil Major Basic Protein; Monoclonal Antibody; Eosinophil Major Basic Protein (Azide Free); Anti -Eosinophil Major Basic Protein (Azide Free); anti-PRG2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1.B.787
• Specificity: Recognizes human eosinophil major basic protein (14kD) irrespective of the stages of eosinophil activation and can be regarded as a "pan-eosinophil" marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kD) and mature form found in the matrix of the eosinophil large specific granule (13.8kD). Does not crossreact with equine, mouse or rat MBP.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA. No preservative added.

• Applicable Applications for anti-PRG2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot and ELISA.; Dilution: Immunohistochemistry: Fresh frozen (methanol: acetone), FFPE: treat with 0.1% trypsin before blocking.
• Immunogen: Major Basic Protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-PRG2 antibody

Eosinophil major basic protein is felt to play an important role in eosinophil function and perhaps endothelial injury in hypereosinophilic states. Proteoglycan 2, bone marrow, also known as PRG2, is a human gene. The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. A 117-residue protein that predominates in eosinophil granules. It is a potent enzyme against helminths and is toxic towards bacteria and mammalian cells in vitro. The eosinophil major basic protein also causes the release of histamine from mast cells and basophils, activates neutrophils and alveolar macrophages, and is directly implicated in epithelial cell damage, exfoliation and bronchospasm in asthma. Structurally the major basic protein (MBP) is similar to lectins (sugar-binding proteins), and has a fold similar to that seen in C-type lectins. However, unlike other C-type lectins (those that bind various carbohydrates in the presence of calcium), MBP does not bind either calcium or any of the other carbohydrates that this family recognize. Instead, MBP recognizes heparan sulfate proteoglycans. The structure of MBP is available from the Protein Data Bank, accession number 1h8u[2] and 2brs[3].

Product Categories/Family for anti-PRG2 antibody

Antibodies; Abs to Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 306839
• NCBI GeneID: 5553
• UniProt Accession #: P13727; P81448; Q14227; Q6ICT2
• Molecular Weight: 25,206 Da
• NCBI Official Full Name: eosinophil major basic protein
• NCBI Official Synonym Full Names: proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• NCBI Official Symbol: PRG2
• NCBI Official Synonym Symbols: MBP; BMPG; MBP1; MGC14537
• NCBI Protein Information: bone marrow proteoglycan; OTTHUMP00000234774; OTTHUMP00000234775; OTTHUMP00000234776; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein
• UniProt Protein Name: Bone marrow proteoglycan
• Protein Family: Bone marrow proteoglycan
• UniProt Gene Name: PRG2
• UniProt Synonym Gene Names: MBP
• UniProt Entry Name: PRG2_HUMAN

NCBI Description

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. [provided by RefSeq]

Uniprot Description

PRG2: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.

Protein type: Secreted; Vesicle; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: transport vesicle; extracellular region

Molecular Function: heparin binding; carbohydrate binding

Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; defense response to bacterium; immune response; defense response to nematode