Western Blot (WB)
(The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein Lane 2.: ENO2 (Gamma-enolase) recombinant protein Lane 3.: ENO3 (Beta-enolase) recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: HeLa cell lysate )
Mouse anti-Human ENO1 Monoclonal Antibody | anti-ENO1 antibody
ENO1, 2 antibody
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein Lane 2.: ENO2 (Gamma-enolase) recombinant protein Lane 3.: ENO3 (Beta-enolase) recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: HeLa cell lysate )
Western Blot (WB)
(The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein Lane 2.: ENO2 (Gamma-enolase) recombinant protein Lane 3.: ENO3 (Beta-enolase) recombinant protein The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: HeLa cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
Uniprot Description
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
Research Articles on ENO1
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Product Notes
The ENO1 eno1 (Catalog #AAA200423) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENO1, 2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENO1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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