Mouse anti-Human Endostatin Monoclonal Antibody | anti-Col18a1 antibody

Endostatin, Human, mAb 1837-46

Cat. Num. AAA584419

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Synonyms: Endostatin; Monoclonal Antibody; Human; mAb 1837-46; anti-Col18a1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 184

• Applicable Applications for anti-Col18a1 antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA), P, Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For staining of paraffin sections antigen retrieval (microwave irradiation (2x7 minutes) in citrate buffer) is advised.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Col18a1 antibody

Product Description: Monoclonal antibody 1837-46 reacts with endostatin, a 20 kDa C-terminal fragment of collagen XVIII. Endostatin is able to evoke non-uniform response for proliferation, cell mount and migration of entothelial cells, with different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce acquired drug resistance and has therefore become a potent new therapy strategy in solid neoplasias. This therapy appear to have high potential not only for the treatment of gliomas, the most common brain tumours, but also of other tumours. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn2+ binding and elastase processing. Widespread endostatin expression was found in elastic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.

References

1. Deininger, M et al; Accumulation of endostatin/collagenXVIII in brains of patients who died with cerebral malaria. J Neuroimmunol 2002, 131: 216 2. Deininger, M et al; Aberrant neuronal and paracellular deposition of endostatin in brains of patients with alzheimer's disease. J Neurosci 2002, 22: 10621

NCBI and Uniprot Product Information

• NCBI GI #: 7739777
• NCBI GeneID: 12822
• Molecular Weight: 134,204 Da
• NCBI Official Full Name: endostatin, partial
• NCBI Official Synonym Full Names: collagen, type XVIII, alpha 1
• NCBI Official Symbol: Col18a1
• NCBI Protein Information: collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; procollagen, type XVIII, alpha 1
• UniProt Protein Name: Collagen alpha-1(XVIII) chain
• Protein Family: Collagen
• UniProt Gene Name: Col18a1
• UniProt Entry Name: COIA1_MOUSE

Uniprot Description

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; collagen; extracellular region; basement membrane

Molecular Function: identical protein binding; metal ion binding; structural molecule activity

Biological Process: extracellular matrix organization and biogenesis; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; positive regulation of cell migration