Typical Testing Data/Standard Curve (for reference only)
Mouse Endostatin ELISA Kit | COL18A1 elisa kit
Mouse Endostatin ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
Endostatin; Mouse Endostatin ELISA Kit; Collagen alpha-1(XVIII) chain; COL18A1 elisa kit
Reactivity
Mouse
Specificity
The Mouse Endostatin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Endostatin proteins within the range of 156 pg/ml - 10000 pg/ml.
Sequence Length
1774
Applicable Applications for COL18A1 elisa kit
Sandwich ELISA (EIA)
Samples
Cell culture supernatants, serum, plasma
Sensitivity
< 10 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COL18A1 elisa kit
Principle of the Assay: The MyBioSource' Mouse Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Endostatin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
Background/Introduction: Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
134,204 Da
NCBI Official Full Name
Collagen alpha-1(XVIII) chain
NCBI Official Synonym Full Names
collagen, type XVIII, alpha 1
NCBI Official Symbol
Col18a1
NCBI Protein Information
collagen alpha-1(XVIII) chain
UniProt Protein Name
Collagen alpha-1(XVIII) chain
Protein Family
UniProt Gene Name
Col18a1
UniProt Entry Name
COIA1_MOUSE
Uniprot Description
COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: basement membrane; collagen; extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix
Molecular Function: identical protein binding; metal ion binding; structural molecule activity
Biological Process: angiogenesis; cell adhesion; endothelial cell morphogenesis; extracellular matrix organization and biogenesis; positive regulation of cell migration; positive regulation of cell proliferation
Research Articles on COL18A1
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Product Notes
The Mouse COL18A1 col18a1 (Catalog #AAA8244667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244667 ELISA Kit recognizes Mouse COL18A1. AAA Biotech's Endostatin can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the COL18A1 col18a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endostatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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