Hamster EMR1 Monoclonal Antibody | anti-EMR1 antibody

HAMSTER ANTI HUMAN EMR1

Cat. Num. AAA224576

• Gene Names: EMR1; TM7LN3
• Applications: Flow Cytometry, Functional Assay
• Synonyms: EMR1; Monoclonal Antibody; HAMSTER ANTI HUMAN EMR1; anti-EMR1 antibody

• Host: Hamster
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: A10
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 867

• Applicable Applications for anti-EMR1 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Fibroblast cell line ARH102 expressing CD97-EMR1 (EGF1-6); Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human eosinophils stained with Hamster anti Human EMR1 (MBS224708) followed by Goat anti Hamster IgG:Biotin (MBS235098) and Streptavidin:RPE (MBS238414))

Testing Data #2

(Human eosinophils stained with Hamster anti Human EMR1:RPE (MBS224415) )

Related Product Information for anti-EMR1 antibody

Hamster anti Human EMR1 antibody, clone A10 recognizes human EMR1 (EGF-like module containing, mucin-like, hormone receptor-like 1). EMR1 is a seven-span transmembrane protein which is a member of the EGF-TM7 family of receptors. EMR1 is the human ortholog of murine F4/80 but unlike F4/80, the expression of EMR1 in humans is restricted to eosinophils and is not expressed on macrophage populations. The detailed structure and function of EMR1 is still unknown.

NCBI and Uniprot Product Information

• NCBI GI #: 372266078
• NCBI GeneID: 2015
• NCBI Accession #: NP_001243181.1
• NCBI GenBank Nucleotide #: NM_001256252.1
• UniProt Accession #: Q14246; Q2I7G5; Q6ZMN0; Q8NGA7; A6NHV2; B7Z486; B7Z489; E7EPX9; E9PD45; H9KV79
• Molecular Weight: 82,771 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 1 isoform 2
• NCBI Official Synonym Full Names: egf-like module containing, mucin-like, hormone receptor-like 1
• NCBI Official Symbol: EMR1
• NCBI Official Synonym Symbols: TM7LN3
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; egf-like module containing, mucin-like, hormone receptor-like sequence 1
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 1
• UniProt Gene Name: EMR1
• UniProt Synonym Gene Names: TM7LN3
• UniProt Entry Name: EMR1_HUMAN

NCBI Description

This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function: G-protein coupled receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell adhesion