Western Blot (WB)
(Western blot analysis of 1) 293T,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000.)
Mouse eIF4A1 Monoclonal Antibody | anti-eIF4A1 antibody
eIF4A1 Mouse mAb
Gene Names
EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Synonyms
eIF4A1; Monoclonal Antibody; eIF4A1 Mouse mAb; DDX2; eIF 4A I; Eukaryotic initiation factor 4A-1; ATP-dependent RNA helicase eIF4A-1;DDX2A; anti-eIF4A1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Form/Format
PBS with 0.02% sodium azide and 50% glycerol pH7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-eIF4A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1,000-3,000
IF: 1:100-200
IHC: 1:100-200
IF: 1:100-200
IHC: 1:100-200
Species
Human
Immunogen
Synthetic Peptide
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of 1) 293T,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000.)
Western Blot (WB)
(Western blot analysis of 1) 293T,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000.)
Immunofluorescence (IF)
(IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100.)
Immunofluorescence (IF)
(IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1(EM1136)Mouse mAb diluted at 1:200.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1(EM1136)Mouse mAb diluted at 1:200.)
Related Product Information for anti-eIF4A1 antibody
The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA-this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.
Product Categories/Family for anti-eIF4A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,548 Da
NCBI Official Full Name
eukaryotic initiation factor 4A-I isoform 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4A1
NCBI Official Symbol
EIF4A1
NCBI Official Synonym Symbols
DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
NCBI Protein Information
eukaryotic initiation factor 4A-I; ATP-dependent RNA helicase eIF4A-1; eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1
UniProt Protein Name
Eukaryotic initiation factor 4A-I
Protein Family
UniProt Gene Name
EIF4A1
UniProt Synonym Gene Names
DDX2A; EIF4A; eIF-4A-I; eIF4A-I
UniProt Entry Name
IF4A1_HUMAN
Similar Products
Product Notes
The eIF4A1 eif4a1 (Catalog #AAA8808581) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The eIF4A1 Mouse mAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's eIF4A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1,000-3,000 IF: 1:100-200 IHC: 1:100-200. Researchers should empirically determine the suitability of the eIF4A1 eif4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eIF4A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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