Western Blot (WB)
(Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1, 000 dilution.)
Mouse anti-Human EIF2A Monoclonal Antibody | anti-EIF2A antibody
EIF2A Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1, 000 dilution. Positive control�� Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:)
Western Blot (WB)
(Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1, 000 dilution. Positive control�� Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
NCBI and Uniprot Product Information
NCBI Description
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM, Mar 2008]
Uniprot Description
EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 3q25.1
Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex
Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding
Biological Process: regulation of translation; ribosome assembly; positive regulation of signal transduction; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation
Research Articles on EIF2A
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Product Notes
The EIF2A eif2a (Catalog #AAA9435285) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the EIF2A eif2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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