Mouse anti-Human EHD2 Monoclonal Antibody | anti-EHD2 antibody

EHD2 (EH Domain Containing 2, FLJ96617, PAST2) (Azide Free) (PE)

Cat. Num. AAA6156218

• Gene Names: EHD2; PAST2
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation
• Synonyms: EHD2; Monoclonal Antibody; EHD2 (EH Domain Containing 2; FLJ96617; PAST2) (Azide Free) (PE); EH-domain Containing Protein 2; anti-EHD2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 10C43
• Specificity: Recognizes human EHD2 at ~61kD.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 543

• Applicable Applications for anti-EHD2 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: ELISA: 1ug/ml-3ng/ml; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to a portion of amino acids within aa365-428 of human EHD20 (GST tag) (NP_055416).
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-EHD2 antibody

Antibodies; Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21361462
• NCBI GeneID: 30846
• NCBI Accession #: NP_055416
• NCBI GenBank Nucleotide #: NP_055416.2
• UniProt Accession #: Q9NZN4
• NCBI Official Full Name: EH domain-containing protein 2
• NCBI Official Synonym Full Names: EH domain containing 2
• NCBI Official Symbol: EHD2
• NCBI Official Synonym Symbols: PAST2
• NCBI Protein Information: EH domain-containing protein 2
• UniProt Protein Name: EH domain-containing protein 2
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD2
• UniProt Synonym Gene Names: PAST2
• UniProt Entry Name: EHD2_HUMAN

NCBI Description

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: recycling endosome membrane; perinuclear region of cytoplasm; plasma membrane; endosome membrane; caveola; nucleus

Molecular Function: protein domain specific binding; protein binding; nucleic acid binding; GTP binding; hydrolase activity; calcium ion binding; ATP binding

Biological Process: metabolic process; endocytosis; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; blood coagulation