Rabbit EHD1 Monoclonal Antibody | anti-EHD1 antibody

EHD1 Rabbit mAb

Cat. Num. AAA4752713

• Gene Names: EHD1; PAST; PAST1; H-PAST; HPAST1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: EHD1; Monoclonal Antibody; EHD1 Rabbit mAb; EH domain-containing protein 1; PAST; PAST1; H-PAST; HPAST1; anti-EHD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R04-8B5
• Specificity: Highly expressed in testis.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-EHD1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:10,000; ICC/IF: 1:20-1:50; FC/FACS: 1:100-1:500; IP: 1:20-1:50
• Immunogen: A synthetic peptide of human EHD1
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cell membrane, Cell projection, Cilium, Endosome, Membrane
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of EHD1 in K562,Rat Brain,C6,3T3 cell lysates using EHD1 Rabbit mAb(1:1000 diluted). Predicted band size:61kDa. Observed band size:61kDa.)

Related Product Information for anti-EHD1 antibody

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (PubMed:24019528). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:15020713, PubMed:17233914, PubMed:20801876). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (PubMed:25686250).

NCBI and Uniprot Product Information

• NCBI GI #: 30240932
• NCBI GeneID: 10938
• NCBI Accession #: NP_006786.2
• NCBI GenBank Nucleotide #: NM_006795.3
• UniProt Accession #: Q9H4M9; O14611; Q2M3Q4; Q9UNR3
• Molecular Weight: 60,627 Da
• NCBI Official Full Name: EH domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: EH-domain containing 1
• NCBI Official Symbol: EHD1
• NCBI Official Synonym Symbols: PAST; PAST1; H-PAST; HPAST1
• NCBI Protein Information: EH domain-containing protein 1; testilin; PAST homolog 1
• UniProt Protein Name: EH domain-containing protein 1
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD1
• UniProt Synonym Gene Names: PAST; PAST1; hPAST1
• UniProt Entry Name: EHD1_HUMAN

NCBI Description

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

Uniprot Description

EHD1: Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.

Protein type: Calcium-binding; Vesicle

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: membrane; recycling endosome membrane; early endosome membrane; plasma membrane; lipid particle; endosome membrane

Molecular Function: protein binding; GTP binding; calcium ion binding; ATP binding; Rab GTPase binding

Biological Process: intracellular protein transport; cholesterol homeostasis; endocytosis; blood coagulation; endocytic recycling; neurite development; protein homooligomerization