Mouse anti-Human EGFR Monoclonal Antibody | anti-EGFR antibody

EGFR (EGFR1) Antibody

Cat. Num. AAA440119

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: EGFR; Monoclonal Antibody; EGFR (EGFR1) Antibody; anti-EGFR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Specificity: Reacts with EGFR of human origin
• Form/Format: 100 ug/ml of mouse monoclonal IgG1 in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.100 mg/ml (varies by lot)
• Sequence Length: 10

• Applicable Applications for anti-EGFR antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western blotting starting dilution 1:200.
• Origin: EGFR (EGFR1) is a mouse monoclonal IgG2b generated by immunizing mice with the human epidermoid carcinoma line A431.
• Immunogen: Human epidermoid carcinoma line A431; epitope mapped between aa 6-273 of human EGFR
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-EGFR antibody

The EFGR family is a group of four structurally similar tyrosine kinases (EGFR, HER2/neu, ErbB-3, and ErbB-4) that dimerize on binding with a number of ligands, including EGF and transforming growth factor alpha (1&2). The 170 kDa epidermal growth factor receptor binds EGF which activates a tyrosine-specific protein kinase activity intrinsic to the receptor (3&4). Epidermal growth factor receptor overexpression is pronounced in virtually all squamous carcinomas and is also found in > or = 65% of large cell and adenocarcinomas. It is not expressed in situ by small cell lung carcinoma. Overexpression of EGFR is one of the earliest and most consistent abnormalities in bronchial epithelium of high-risk smokers (1).

References

1.) Weyergang A, Selbo PK, Berg K. 2007. Y1068 phosphorylation is the most sensitive target of disulfonated tetraphenylporphyrin-based photodynamic therapy on epidermal growth factor receptor. Biochem Pharmacol. Jul 15;74(2):226-35.

1. Franklin WA, Veve R, Hirsch FR, Helfrich BA, Bunn PA Jr. Epidermal growth factor receptor family in lung cancer and premalignancy. Semin Oncol 2002 Feb;29(1 Suppl 4):3-14.2. Yarden Y. The EGFR family and its ligands in human cancer, signalling mechanisms and therapeutic opportunities. Eur J Cancer 2001 Sep: 37 Suppl 4: S3-8.3. Reynolds F.H. Jr., Todaro G.J., Fryling C., and Stephenson J.R. Human transforming growth factors induces tyrosine phosphorylation of EGR receptors. Nature 1981 292: 259-262.4. Chen W.S., Lazar C.S., Poenie M, Tsien R.Y., Gill G.N., Rosenfeld M.G. Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor. Nature 1987 328: 820-823.

NCBI and Uniprot Product Information

• NCBI GI #: 8815559
• UniProt Accession #: Q9P2Z9
• Molecular Weight: 1,020 Da
• NCBI Official Full Name: epidermal growth factor receptor, partial
• UniProt Protein Name: Epidermal growth factor receptor
• Protein Family: Epidermal growth factor receptor
• UniProt Gene Name: c-erbB proto-oncogene
• UniProt Entry Name: Q9P2Z9_HUMAN