Western Blot (WB)
(Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179825).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEA1 )
Rabbit EEA1 Monoclonal Antibody | anti-EEA1 antibody
Anti-EEA1 Rabbit Monoclonal Antibody
Gene Names
EEA1; MST105; ZFYVE2; MSTP105
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Affinity-chromatography
Synonyms
EEA1; Monoclonal Antibody; Anti-EEA1 Rabbit Monoclonal Antibody; Early endosome antigen 1; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2; ZFYVE2; anti-EEA1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Purity/Purification
Affinity-chromatography
Form/Format
Liquid
Sequence Length
1,411
Applicable Applications for anti-EEA1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
ICC/IF: 1:50-1:200
Immunogen
A synthesized peptide derived from human EEA1
Subcellular Localization
Cytoplasm. Early endosome membrane; Peripheral membrane protein.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179825).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEA1 )
Western Blot (WB)
(Western blot analysis of EEA1 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179825).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EEA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EEA1 )
Related Product Information for anti-EEA1 antibody
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Product Categories/Family for anti-EEA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162466 MW
NCBI Official Full Name
early endosome antigen 1
NCBI Official Synonym Full Names
early endosome antigen 1
NCBI Official Symbol
EEA1
NCBI Official Synonym Symbols
MST105; ZFYVE2; MSTP105
NCBI Protein Information
early endosome antigen 1
UniProt Protein Name
Early endosome antigen 1
Protein Family
UniProt Gene Name
EEA1
UniProt Synonym Gene Names
ZFYVE2
Uniprot Description
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Research Articles on EEA1
Similar Products
Product Notes
The EEA1 eea1 (Catalog #AAA179825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-EEA1 Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEA1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). WB: 1:500-1:2000 ICC/IF: 1:50-1:200. Researchers should empirically determine the suitability of the EEA1 eea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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