Western Blot (WB)
(Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human EBAG9 Monoclonal Antibody | anti-EBAG9 antibody
Recombinant Anti-EBAG9 Antibody, Rabbit Monoclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Western Blot (WB)
(Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-EBAG9 rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:24 kDaObserved band size:37 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(EBAG9 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-EBAG9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EBAG9 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 37 kDa)
Immunoprecipitation (IP)
(EBAG9 was immunoprecipitated using:Lane A:0.5 mg THP-1 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-EBAG9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EBAG9 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 24 kDaObserved band size: 37 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013]
Uniprot Description
RCAS1: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1- beta converting enzyme (ICE)-like proteases. Homodimer. By estrogen. Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.
Protein type: Membrane protein, integral; Apoptosis
Chromosomal Location of Human Ortholog: 8q23
Cellular Component: Golgi membrane; Golgi apparatus; focal adhesion; plasma membrane; integral to membrane; secretory granule
Molecular Function: apoptotic protease activator activity
Biological Process: apoptosis; regulation of cell growth
Research Articles on EBAG9
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Product Notes
The EBAG9 ebag9 (Catalog #AAA8107212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-EBAG9 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EBAG9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the EBAG9 ebag9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBAG9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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