Rabbit Olfactomedin 4 ELISA Kit | OLFM4 elisa kit

Rabbit Olfactomedin 4 ELISA Kit

Cat. Num. AAA729500

• Gene Names: OLFM4; GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
• Reactivity: Rabbit
• Synonyms: Olfactomedin 4; Rabbit Olfactomedin 4 ELISA Kit; OLFM4 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This OLFM4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit OLFM4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OLFM4 elisa kit

Principle of the assay: OLFM4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OLFM4 antibody and an OLFM4-HRP conjugate. The assay sample and buffer are incubated together with OLFM4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OLFM4 concentration since OLFM4 from samples and OLFM4-HRP conjugate compete for the anti-OLFM4 antibody binding site. Since the number of sites is limited, as more sites are occupied by OLFM4 from the sample, fewer sites are left to bind OLFM4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLFM4 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OLFM4 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 32313593
• NCBI GeneID: 10562
• NCBI Accession #: NP_006409.3
• NCBI GenBank Nucleotide #: NM_006418.4
• Molecular Weight: 57,280 Da
• NCBI Official Full Name: olfactomedin-4
• NCBI Official Synonym Full Names: olfactomedin 4
• NCBI Official Symbol: OLFM4
• NCBI Official Synonym Symbols: GC1; OLM4; OlfD; GW112; hGC-1; hOLfD; UNQ362; bA209J19.1
• NCBI Protein Information: olfactomedin-4; antiapoptotic protein GW112; G-CSF-stimulated clone 1 protein
• UniProt Protein Name: Olfactomedin-4
• Protein Family: Olfactomedin
• UniProt Gene Name: OLFM4
• UniProt Synonym Gene Names: GW112; UNQ362/PRO698; OLM4; hGC-1
• UniProt Entry Name: OLFM4_HUMAN

NCBI Description

This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]

Uniprot Description

OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: extracellular space; specific granule; azurophil granule; mitochondrion; perinuclear region of cytoplasm; plasma membrane; secretory granule

Molecular Function: protein homodimerization activity; cadherin binding; catalytic activity

Biological Process: regulation of apoptosis; metabolic process; negative regulation of immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; regulation of phagocytosis; cell adhesion; protein homooligomerization