Rat anti-Human, Mouse E-selectin Monoclonal Antibody | anti-SELE antibody

Rat anti E-selectin

Cat. Num. AAA570119

• Gene Names: SELE; ELAM; ESEL; CD62E; ELAM1; LECAM2
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: E-selectin; Monoclonal Antibody; Rat anti E-selectin; anti-SELE antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: UZ4
• Specificity: UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectin domain of both murine and Human E-selectin.
• Form/Format: Each vial contains 100ul 1 mg/ml purified monoclonal antibody in PBS containing 0,09% sodium azide.

• Applicable Applications for anti-SELE antibody: Flow Cytometry (FC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: UZ4 is suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry (dilution 1:500). To analyze E-selectin protein in frozen sections we refer to the anti E-selectin monoclonal antibodies UZ5, UZ6, and UZ7. For a functional UZ4 antibody that inhibits leucocyte adhesion to murine and Human activated endothelial cells refer to the E-selectin antibody for in vivo use which does not contain azide.
• Source Note: UZ4 is a Rat monoclonal IgM antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a Lewis Rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-SELE antibody

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Product Categories/Family for anti-SELE antibody

Cell adhesion; Cell adhesion; Immunology

References

Hallmann, R., Zimmermann, U., Sorokin, L. M., Needham, L., and Von der Mark, K. (1995). Adhesion of leukocytes to the inflammed endothelium, Scand J Rheumatol Suppl 101, 107-9. Austrup, F., Vestweber, D., Borges, E., Lohning, M., Brauer, R., Herz, U., Renz, H., Hallmann, R., Scheffold, A., Radbruch, A., and Hamann, A. (1997). P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues, Nature 385, 81-3. Borges, E., Tietz, W., Steegmaier, M., Moll, T., Hallmann, R., Hamann, A., and Vestweber, D. (1997). P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migRation into inflamed skin, J Exp Med 185, 573-8. Tietz, W., Allemand, Y., Borges, E., von Laer, D., Hallmann, R., Vestweber, D., and Hamann, A. (1998). CD4+ T cells migRate into inflamed skin only if they express ligands for E- and P-selectin, J Immunol 161, 963-70. Alferink, J., Tafuri, A., Vestweber, D., Hallmann, R., Hammerling, G. J., and Arnold, B. (1998). Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking, Science 282, 1338-41. Wolf, D., Hallmann, R., Sass, G., Sixt, M., Kusters, S., Fregien, B., Trautwein, C., and Tiegs, G. (2001). TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis, J Immunol 166, 1300-7. Hammel, M., Weitz-Schmidt, G., Krause, A., Moll, T., Vestweber, D., Zerwes, H. G., and Hallmann, R. (2001). Species-specific and conserved epitopes on Mouse and Human E-selectin important for leukocyte adhesion, Exp Cell Res 269, 266-74. Sunderkotter, C., Seeliger, S., Schonlau, F., Roth, J., Hallmann, R., Luger, T. A., Sorg, C., and Kolde, G. (2001). Different pathways leading to cutaneous leukocytoclastic vasculitis in mice, Exp Dermatol 10, 391-404. Kneilling, M., Reinhard, M., Hültner, L., Schönberger, T., Fuchs, K., Schaller, M., Bukala, D., Massberg, S., Sander, C.A., Braumüller, H., Eichner, M., Maier, K.L., Hallmann, R., Pichler, B.J., Haubner, R., Gawaz, M., Pfeffer, K., Biedermann, T., Röcken, M. (2009). Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation. Blood, 114, 1696-1706.

NCBI and Uniprot Product Information

• NCBI GI #: 187960042
• NCBI GeneID: 6401
• NCBI Accession #: NP_000441.2
• NCBI GenBank Nucleotide #: NM_000450.2
• UniProt Accession #: P16581; P16111; A2RRD6; Q5TI75; Q6ULR7
• Molecular Weight: 66,655 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: SELE
• NCBI Official Synonym Symbols: ELAM; ESEL; CD62E; ELAM1; LECAM2
• NCBI Protein Information: E-selectin; ELAM-1; OTTHUMP00000032745; OTTHUMP00000032746; OTTHUMP00000032747; OTTHUMP00000032748; OTTHUMP00000032749; endothelial adhesion molecule 1; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• Protein Family: E-selectin
• UniProt Gene Name: SELE
• UniProt Synonym Gene Names: ELAM1
• UniProt Entry Name: LYAM2_HUMAN

NCBI Description

The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq]

Uniprot Description

SELE: Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. Belongs to the selectin/LECAM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; plasma membrane; integral to membrane; coated pit; caveola; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; phospholipase binding; sialic acid binding

Biological Process: positive regulation of leukocyte migration; actin filament-based process; positive regulation of receptor internalization; calcium-mediated signaling; response to lipopolysaccharide; leukocyte migration during inflammatory response; heterophilic cell adhesion; leukocyte adhesion; phospholipase C activation; regulation of inflammatory response; leukocyte tethering or rolling; inflammatory response; blood coagulation; leukocyte migration

Disease: Hypertension, Essential