Mouse anti-Rat E-Selectin Monoclonal Antibody | anti-SELE antibody

Anti-E-Selectin Antibody, Mouse Monoclonal

Cat. Num. AAA8112670

• Reactivity: Rat
• Applications: ELISA
• Purity: Protein A
• Synonyms: E-Selectin; Monoclonal Antibody; Anti-E-Selectin Antibody; Mouse Monoclonal; E-Selectin/CD62e/SELE Antibody; Mouse MAb; selectin E; anti-SELE antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 01
• Specificity: Rat E-Selectin/CD62e/SELE.; No cross-reactivity in ELISA with Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SELE antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Rat E-Selectin/CD62e/SELE protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat E-Selectin/CD62e/SELE (rR E-Selectin/CD62e/SELE). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-SELE antibody

E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. E-selectin exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-selectin recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-selectin supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. E-selectin regulates adhesive interactions between certain blood cells and endothelium. The soluble form of E selectin (sE-selectin) is a marker of endothelial activation, and has a potential role in the pathogenesis of cardiovascular disease as raised levels have been found in hypertension, diabetes and hyperlipidemia, although its association in established atherosclerosis disease and its value as a prognostic factor is more controversial. soluble E-selectin is inversely associated with the muscular component of the left ventricle, thereby suggesting that the lack of such a reparative factor may be associated with cardiac remodeling in end-stage renal disease (ESRD) patients. Besides, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

References

Roldn V, et al. (2003) Soluble E-selectin in cardiovascular disease and its risk factors. A review of the literature. Thromb Haemost. 90(6): 1007-20.Kawase J, et al. (2009) Increase in E-selectin expression in umbilical vein endothelial cells by anticancer drugs and inhibition by cimetidine. Oncol Rep. 22(6): 1293-7.Matsumoto K, et al. (2010) Soluble adhesion molecule E-selectin predicts cardiovascular events in Japanese patients with type 2 diabetes mellitus. Metabolism. 59(3): 320-4.Stancanelli B, et al. (2010) Soluble e-selectin is an inverse and independent predictor of left ventricular wall thickness in end-stage renal disease patients. Nephron Clin Pract. 114(1): c74-80.

NCBI and Uniprot Product Information

• NCBI GI #: 1346437
• NCBI GeneID: 25544
• UniProt Accession #: P98105
• Molecular Weight: 60,080 Da
• NCBI Official Full Name: E-selectin
• NCBI Official Synonym Full Names: selectin E
• NCBI Official Symbol: Sele
• NCBI Protein Information: E-selectin; ELAM-1; LECAM2; selectin, endothelial cell; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2
• UniProt Protein Name: E-selectin
• Protein Family: E-selectin
• UniProt Gene Name: Sele
• UniProt Synonym Gene Names: Elam-1; ELAM-1; LECAM2
• UniProt Entry Name: LYAM2_RAT

NCBI Description

facilitates recruitment of leukocytes into sites of inflammation; plays a role in cell adhesion [RGD, Feb 2006]

Uniprot Description

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis ().