Immunohistochemistry (IHC)
(IHC (paraffin) of human colon using Cytokeratin 14 Antibody)
Mouse anti-Human Cytokeratin 14 Monoclonal Antibody | anti-KRT14 antibody
Cytokeratin 14 Antibody
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
Cytokeratin 14; Monoclonal Antibody; Cytokeratin 14 Antibody; anti-KRT14 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
IHC555
Form/Format
Liquid
Sequence Length
471
Applicable Applications for anti-KRT14 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes
Dilution Range: 1:100 - 1:200
Buffer
Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
IHC Positive Control
Squamous Cell Carcinoma
IHC Suggested Protocol
1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide.
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C.
3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes.
4. Block – Block with peroxidase solution for 5 minutes at RT.
5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT.
6. Secondary Ab - Incubate for 20 - 30 minutes at RT.
7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT.
8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds.
9. Dehydrate and apply coverslip.
(Each researcher should optimize the protocol for their experimental model system.)
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
Immunohistochemistry (IHC)
(IHC (paraffin) of human colon using Cytokeratin 14 Antibody)
Immunohistochemistry (IHC)
(IHC (paraffin) of human colon using Cytokeratin 14 Antibody)
Related Product Information for anti-KRT14 antibody
CK14 (Cytokeratin 14) is part of the type I keratin family proteins, and can be found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 antibodies can be useful in identifying squamous cell carcinomas from other epithelial tumors, and for classifying metaplastic breast carcinomas.
NCBI and Uniprot Product Information
Uniprot Description
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Similar Products
Product Notes
The Cytokeratin 14 krt14 (Catalog #AAA346009) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin 14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin 14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin. Dilution Range: 1:100 - 1:200. Researchers should empirically determine the suitability of the Cytokeratin 14 krt14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
