Mouse anti-General DMPO Nitrone Adduct Monoclonal Antibody | anti-DMPO antibody
DMPO Nitrone Adduct Monoclonal Antibody
Reactivity
General
Applications
Western Blot, ELISA, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography.
Synonyms
DMPO Nitrone Adduct; Monoclonal Antibody; DMPO Nitrone Adduct Monoclonal Antibody; anti-DMPO antibody
Host
Mouse
Reactivity
General
Clonality
Monoclonal
Isotype
IgG1
Clone Number
N1664A
Specificity
This antibody recognizes DMPO, DMPOoctanoicacid, DMPO-protein adducts, andDMPO-DNA adducts. It does not crossreactwith non-adducted proteins or DNA.Species independent.
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
100ug in PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Applicable Applications for anti-DMPO antibody
Western Blot, ELISA, Immunocytochemistry
Application Notes
Immunoblotting: use at 1-0ug/ml. A bandof ~90kDa is detected.
ELISA: use at 1-10ug/ml
Immunocytochemistry: use at10ug/ml.Positive control: DMPO nitrone adducts ofmetmyoglobin.
ELISA: use at 1-10ug/ml
Immunocytochemistry: use at10ug/ml.Positive control: DMPO nitrone adducts ofmetmyoglobin.
Antigen
5,5-dimethyl-2-(8-octanoicacid)-1-pyrrolone-N-oxide conjugated toovalbumin.
Dilution Instruction
Dilute in PBS or medium which is identicalto that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C.
Related Product Information for anti-DMPO antibody
Free radicals are highly reactive andgenerally short-lived species. Hence, moststudies of free radicals have beenrestricted to analysis of end productsformed following their interaction withcellular biomolecules. One technology thathas permitted direct study of free radicalsis electron spin resonance (ESR). For veryshort lived radicals, spin traps have beenemployed to generate longer lived radicalsthat are more amenable to ESR analysis.The most widely used of these spin trapsis 5,5-dimethyl-1-pyrroline-N-oxide(DMPO). The reaction of DMPO with aprotein radical gives rise to a nitroxideradical which can be disproportionate tohydrozylamine and nitrone adducts. Ofthese species, only the nitrone adduct canbe considered stable.
Product Categories/Family for anti-DMPO antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DMPO (Catalog #AAA190617) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DMPO Nitrone Adduct Monoclonal Antibody reacts with General and may cross-react with other species as described in the data sheet. AAA Biotech's DMPO Nitrone Adduct can be used in a range of immunoassay formats including, but not limited to, Western Blot, ELISA, Immunocytochemistry. Immunoblotting: use at 1-0ug/ml. A bandof ~90kDa is detected. ELISA: use at 1-10ug/ml Immunocytochemistry: use at10ug/ml.Positive control: DMPO nitrone adducts ofmetmyoglobin. Researchers should empirically determine the suitability of the DMPO for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DMPO Nitrone Adduct, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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