Related Product Information for anti-DLL1 antibody
Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains, acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N-or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.
Product Categories/Family for anti-DLL1 antibody
References
Dyczynska E, et al. (2007) Proteolytic processing of delta-like 1 by ADAM proteases. J Biol Chem. 282(1): 436-44.Sun D, et al. (2008) The role of Delta-like 1 shedding in muscle cell self-renewal and differentiation. J Cell Sci. 121(Pt 22): 3815-23.Srensen I, et al. (2009) DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 113(22): 5680-8.
NCBI Description
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]
Uniprot Description
DLL1: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Interacts with Notch receptors. Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Protein type: Cell development/differentiation; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6q27
Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytoplasmic vesicle
Molecular Function: protein binding; calcium ion binding; Notch binding
Biological Process: regulation of cell adhesion; Notch signaling pathway; negative regulation of myeloid cell differentiation; negative regulation of interleukin-10 production; Notch receptor processing; cell fate determination; compartment specification; somite specification; cell-cell signaling; negative regulation of auditory receptor cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; determination of left/right symmetry; cell differentiation; inner ear development; positive regulation of Notch signaling pathway
Product Notes
The DLL1 dll1 (Catalog #
AAA8105539) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-DLL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA) (Cap). ELISA (Cap): This antibody will detect Human DLL1/Delta-like 1 in ELISA pair set. In a sandwich ELISA, it can be used as capture antibody when paired with. Researchers should empirically determine the suitability of the DLL1 dll1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"DLL1, Monoclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
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opening. Please be advised that
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