Mouse Desmoglein 1,2 Monoclonal Antibody | anti-DSG1 antibody

Desmoglein 1,2

Cat. Num. AAA606413

• Gene Names: DSG1; DG1; DSG; CDHF4; PPKS1; SPPK1
• Reactivity: Bovine, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Desmoglein 1; 2; Monoclonal Antibody; Anti -Desmoglein 1; anti-DSG1 antibody

• Host: Mouse
• Reactivity: Bovine, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5F189
• Specificity: Recognizes bovine Desmoglein 1,2. Does not react with cells containing plaque-bearing junctions of non-desmosomal type. Species Crossreactivity: human, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile dH2O.

• Applicable Applications for anti-DSG1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot.; Dilution: Immunohistochemistry (Frozen, paraffin): 1:10 with PBS, pH 7.4; Requires microwave treatment. Incubation Time 1 h at RT; extended with paraffin.
• Immunogen: "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis. Epitope: localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-DSG1 antibody

Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein and desmocollin. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmoglein-1 is synthesized exclusively in the suprabasal layers. The expression of desmoglein-2 varies in the basal layers, depending on the certain types of epidermis.

Product Categories/Family for anti-DSG1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119703744
• NCBI GeneID: 1828
• NCBI Accession #: NP_001933.2
• NCBI GenBank Nucleotide #: NM_001942.2
• UniProt Accession #: Q02413; Q712V6
• Molecular Weight: 113,748 Da
• NCBI Official Full Name: desmoglein-1 preproprotein
• NCBI Official Synonym Full Names: desmoglein 1
• NCBI Official Symbol: DSG1
• NCBI Official Synonym Symbols: DG1; DSG; CDHF4; PPKS1; SPPK1
• NCBI Protein Information: desmoglein-1; DGI; OTTHUMP00000162826; cadherin family member 4; desmosomal glycoprotein 1; pemphigus foliaceus antigen
• UniProt Protein Name: Desmoglein-1
• Protein Family: Desmoglein
• UniProt Gene Name: DSG1
• UniProt Synonym Gene Names: CDHF4
• UniProt Entry Name: DSG1_HUMAN

NCBI Description

Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. The protein encoded by this gene has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. [provided by RefSeq]

Uniprot Description

DSG1: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1); also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; internal side of plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; cytosol; lateral plasma membrane

Molecular Function: toxin binding; gamma-catenin binding; calcium ion binding

Biological Process: intercellular junction assembly; cell-cell adhesion; maternal process involved in pregnancy; protein stabilization; apoptosis; calcium-dependent cell-cell adhesion; homophilic cell adhesion; response to progesterone stimulus; cell structure disassembly during apoptosis

Disease: Palmoplantar Keratoderma I, Striate, Focal, Or Diffuse; Erythroderma, Congenital, With Palmoplantar Keratoderma, Hypotrichosis, And Hyper-ige