Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI. Courtesy of: Dario Alessi Lab, University of Dundee.)
Mouse DENND4C Monoclonal Antibody | anti-DENND4C antibody
DENND4C Antibody: Biotin
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Synonyms
DENND4C; Monoclonal Antibody; DENND4C Antibody: Biotin; Mouse Anti-Human DENND4C Monoclonal IgG1; DENN domain-containing protein 4C antibody; anti-DENND4C antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3E8
Specificity
Detects ~213kDa.
Purity/Purification
Protein G Purified
Form/Format
PBS pH7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DENND4C antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
A 1:1000 dilution was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis.
A 1:1000 dilution was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis.
Immunogen
Full length human recombinant Dennd4c protein
Immunogen Species
Human
Conjugation
Biotin
Tissue Specificity
Widely expressed
Cellular Localization
Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane
Preparation and Storage
Store at -20 degree C
Shipping: Blue Ice
Shipping: Blue Ice
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:100, 1:500, 1:1000 for 1 hour at RT. Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:1000 for 1 hour at RT. Counterstain: DAPI. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP0
(Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody . Three amounts of SMC-610 (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C. Courtesy of: Dario Alessi Lab, University of Dundee.)
Immunoprecipitation (IP0
(Immunoprecipitation analysis using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Tissue: A549, MEF cells. Species: Human, Mouse. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody . Three amounts of SMC-610 (10, 3, and 1 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 or MEF lysate for 2 hours at 4°C. Courtesy of: Dario Alessi Lab, University of Dundee.)
Western Blot (WB)
(Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:1000. Courtesy of: Dario Alessi Lab, University of Dundee.)
Western Blot (WB)
(Western Blot analysis of Human A549, MEF cells showing detection of DENND4C protein using Mouse Anti-DENND4C Monoclonal Antibody, Clone 3E8 . Lane 1: MW ladder. Lane 2: A549 WT. Lane 3: A549 DENND4C KO. Lane 4: MEF. Load: 30 ug. Primary Antibody: Mouse Anti-DENND4C Monoclonal Antibody at 1:1000. Courtesy of: Dario Alessi Lab, University of Dundee.)
Related Product Information for anti-DENND4C antibody
Dennd4c is a guanine nucleotide exchange factor (GEF) for Rab10, and required for GLUT4 translocation (1). It is associated with Alkuraya-Kucinskas Syndrome and Koolen-De Vries Syndrome (2) and interacts with the retromer (3).
Product Categories/Family for anti-DENND4C antibody
References
1. Sano, H. et al. (2011). J Biol Chem. 286(19):16541-5.2. https://www.genecards.org/cgi-bin/carddisp.pl?gene=DENND4C3. McMillan, K. et al. (2016). J Cell Biol. 214(4):4389–412
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
UniProt Protein Name
DENN domain-containing protein 4C
Protein Family
UniProt Gene Name
DENND4C
UniProt Synonym Gene Names
C9orf55; C9orf55B
UniProt Entry Name
DEN4C_HUMAN
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Product Notes
The DENND4C dennd4c (Catalog #AAA8005895) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DENND4C Antibody: Biotin reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DENND4C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:1000 A 1:1000 dilution was sufficient for detection of DENND4C in A549, MEF cells by Western Blot analysis. Researchers should empirically determine the suitability of the DENND4C dennd4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND4C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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