Mouse DDIT3 Monoclonal Antibody | anti-DDIT3 antibody

DDIT3 (7G7) Mouse mAb

Cat. Num. AAA4753858

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: DDIT3; Monoclonal Antibody; DDIT3 (7G7) Mouse mAb; GADD153; MGC4154; anti-DDIT3 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7G7-3B2-2F10
• Specificity: The CHOP mouse monoclonal antibody can recognizeendogenous CHOP proteins.
• Purity/Purification: Affinity purified
• Form/Format: PBS(pH7.4) containing with 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-DDIT3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1,000-2,000; IHC 1:100-200; IF: 1:200
• Immunogen: Synthetic peptide conjugated to KLH.
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cytoplasm, Nucleus
• Preparation and Storage: Store at +4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CHOP Mouse Monoclonal Antibody(2B1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Mouse-brain tissue. 1,CHOP Mouse Monoclonal Antibody(2B1)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP Mouse mAb diluted at 1:200.)

Western Blot (WB)

(Western blot analysis of Mouse Liver Tissue Lysate using CHOP Mouse mAb diluted at 1:2000.)

Related Product Information for anti-DDIT3 antibody

Essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

Product Categories/Family for anti-DDIT3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 304282234
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181986.1
• NCBI GenBank Nucleotide #: NM_001195057.1
• UniProt Accession #: P35638
• Molecular Weight: 19,175 Da
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 2
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; growth arrest and DNA damage-inducible protein GADD153
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: nucleoplasm; late endosome; nucleus; cytosol

Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription factor activity; transcription corepressor activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; unfolded protein response; negative regulation of transcription factor activity; blood vessel maturation; mRNA transcription from RNA polymerase II promoter; response to unfolded protein; response to starvation; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; inhibition of CREB transcription factor; negative regulation of myoblast differentiation; response to DNA damage stimulus

Disease: Myxoid Liposarcoma