Western Blot (WB)
(Western Blot (WB) analysis of DYKDDDDK-Tag (3B9) monoclonal antibody at 1:5000 dilutionLane1:HEK293T whole cell lysate,transfected with pcDNA3. 1-DDDDK-p62-HA. Lane2:HEK293T whole cell lysateLane3:SK-OVCAR3 whole cell lysateLane4:H9C2 whole cell lysate)
Mouse DDDDK Monoclonal Antibody | anti-DDDDK antibody
DDDDK-tag (3B9) Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
DDDDK; Monoclonal Antibody; DDDDK-tag (3B9) Monoclonal Antibody; DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; anti-DDDDK antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Specificity
DDDDK-tag Mouse antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-DDDDK antibody
Western Blot (WB)
Application Notes
WB: 1:5000-1:10,000
Immunogen
Synthetic peptide DDDDK-tag.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -22 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -22 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of DYKDDDDK-Tag (3B9) monoclonal antibody at 1:5000 dilutionLane1:HEK293T whole cell lysate,transfected with pcDNA3. 1-DDDDK-p62-HA. Lane2:HEK293T whole cell lysateLane3:SK-OVCAR3 whole cell lysateLane4:H9C2 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of DYKDDDDK-Tag (3B9) monoclonal antibody at 1:5000 dilutionLane1:HEK293T whole cell lysate,transfected with pcDNA3. 1-DDDDK-p62-HA. Lane2:HEK293T whole cell lysateLane3:SK-OVCAR3 whole cell lysateLane4:H9C2 whole cell lysate)
Related Product Information for anti-DDDDK antibody
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
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Product Notes
The DDDDK (Catalog #AAA3014212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DDDDK-tag (3B9) Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDDDK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:5000-1:10,000. Researchers should empirically determine the suitability of the DDDDK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDDDK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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