Western Blot (WB)
(Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse DACT3 Monoclonal Antibody | anti-DACT3 antibody
DACT3 antibody
Gene Names
DACT3; RRR1; DAPPER3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
DACT3; Monoclonal Antibody; DACT3 antibody; thymus expressed gene 3-like; dapper antagonist of beta-catenin homolog 3 (Xenopus laevis); arginine rich region 1; MGC15476; anti-DACT3 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
2A5
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
629
Applicable Applications for anti-DACT3 antibody
ELISA (EIA), WB (Tissue, Cell lysate), Immunohistochemistry (IHC)
Application Notes
WB: 1:250 ~ 500.
Recommended starting dilution is 1:250.
Recommended starting dilution is 1:250.
Antigen Species
Human
Immunogen
Recombinant human DACT3 (89-202aa) purified from E. coli
Clone Note
Anti-human DACT3 mAb, clone 2A5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human DACT3 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC)
(Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunohistochemistry (IHC)
(Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Related Product Information for anti-DACT3 antibody
DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.
Product Categories/Family for anti-DACT3 antibody
References
Jiang X, et al., (2008) Cancer Cell. 13 (6):529-41; Fisher DA, et al., (2006) Dev Dyn. 235 (9):2620-30
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64,949 Da
NCBI Official Full Name
dapper homolog 3 isoform 1
NCBI Official Synonym Full Names
dishevelled binding antagonist of beta catenin 3
NCBI Official Symbol
DACT3
NCBI Official Synonym Symbols
RRR1; DAPPER3
NCBI Protein Information
dapper homolog 3
UniProt Protein Name
Dapper homolog 3
Protein Family
UniProt Gene Name
DACT3
UniProt Synonym Gene Names
RRR1
Uniprot Description
May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins.
Research Articles on DACT3
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Product Notes
The DACT3 dact3 (Catalog #AAA200125) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DACT3 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DACT3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Tissue, Cell lysate), Immunohistochemistry (IHC). WB: 1:250 ~ 500. Recommended starting dilution is 1:250. Researchers should empirically determine the suitability of the DACT3 dact3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DACT3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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