Mouse D Monoclonal Antibody | anti-D antibody

Anti-D Antibody

Cat. Num. AAA415078

• Applications: Western Blot, Immunoprecipitation
• Synonyms: D; Monoclonal Antibody; Anti-D Antibody; anti-D antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b with lambda light chain
• Clone Number: F-tag-01
• Specificity: Anti-D tag Antibody recognises over-expressed proteins containing D epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
• Form/Format: Liquid
• Concentration: 100ug/100uL (varies by lot)

• Applicable Applications for anti-D antibody: Western Blot (WB), IS, Immunoprecipitation (IP)
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.; Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000; Immunoprecipitation and Immunostaining at dilutions of 1:1000.
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

Rotte, A et al. "Tumor suppressor Ing1b facilitates DNA repair and prevents oxidative stress induced cell death." Apoptosis 19:518-26 (2014). PubMed: 24242916.
Tang, Y et al. "Prognostic significance of KAI1, CD82 in human melanoma and its role in cell migration and invasion through the regulation of ING4." Carcinogenesis 35:86-95 (2014). PubMed: 24130172.
Ji, YJ et al. "EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1." Nat Commun 5:3516 (2014). PubMed: 24662724.
Cherry, AA et al. "Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules." Gene 1:34-44 (2014). PubMed: 24709106.
Antony, S et al. "Characterization of NADPH oxidase 5 expression in human tumors and tumor cell lines with a novel mouse monoclonal antibody." Free Radic Biol Med. 65C:497-508 (2013). DOI: 10.1016/j.freeradbiomed.2013.07.005. Application: Western Blot.
Christova, T et al. "Sox4-mediated Dicer expression is critical for suppression of melanoma cell invasion." Oncogene 32 (17):2131-2139 (2013). DOI: 10.1038/onc.2012.239. Application: Western Blot.
Cheng, Y et al. "Prognostic significance of Fbw7 in human melanoma and its role in cell migration." J Invest Dermatol 133 (7):1794-802 (2013). DOI: 10.1038/jid.2013.58. Application: Western Blot.
Lu, J et al. "ING4 regulates JWA in angiogenesis and their prognostic value in melanoma patients." Br J Cancer 109 (11):2842-1852 (2013). DOI: 10.1038/bjc.2013.670. Application: Western Blot.
Lu, J et al. "JWA inhibits melanoma angiogenesis by suppressing ILK signaling and is an independent prognostic biomarker for melanoma." Carcinogenesis 34 (12):2778-2788 (2013). DOI: 10.1093/carcin/bgt318. Application: Western Blot.
Klein, SL et al. "Conserved structural domains in FoxD4L1, a neural forkhead box transcription factor, are required to repress or activate target genes." PLoS ONE 8:e61845 (2013). PubMed: 23610594. Application: Immunoprecipitation.
Jo, SH et al. "Txnip contributes to impaired glucose tolerance by upregulating the expression of genes involved in hepatic gluconeogenesis in mice." Diabetologia 2013:2723-32 (2013). PubMed: 24037087.
Hwang, YS et al. "The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1." Genes Dev. 27:491-503 (2013). PubMed: 23475958.
Lee, JY et al. "Cell growth defect factor1, chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis." Plant Cell 25:3944-60 (2013). PubMed: 24151298.
Jafarnejad, SM et al. "Sox4-mediated Dicer expression is critical for suppression of melanoma cell invasion." Oncogene 32:2131-9 (2013). PubMed: 22689055.
Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate." Dev Biol 365 (2):353-375 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
Jafarnejad, SM et al. "Role of Sox4 transcription factor in human cutaneous melanoma." Thesis : (2012).
Tran, D et al. "A mutation in VAPB that causes amyotrophic lateral sclerosis also causes a nuclear envelope defect." J Cell Sci 125:2831-6 (2012). PubMed: 22454507.
Li, J et al. "Prognostic significance of BRMS1 expression in human melanoma and its role in tumor angiogenesis." Oncogene 30 (8):896-906 (2011). DOI: 10.1038/onc.2010.470. Application: Western Blot.
Rothenburg, S et al. "Characterization of a ranavirus inhibitor of the antiviral protein kinase PKR." BMC Microbiol. 11:56 (2011). PubMed: 21418572. Application: Western Blot.
Wong, RP et al. "Tumour suppressor ING1b maintains genomic stability upon replication stress." Nucleic Acids Res. 39:3632-42 (2011). PubMed: 21227930.
Christova, T et al. "Lymphoid enhancer factor-1 mediates loading of Pax3 to a promoter harbouring lymphoid enhancer factor-1 binding sites resulting in enhancement of transcription." Int J Biochem & Cell Biol 42 (5):630-640 (2010). DOI: 10.1016/j.biocel.2009.12.006. Application: Western Blot.
Li, J et al. "Cell Cycle Regulator ING4 Is a Suppressor of Melanoma Angiogenesis That Is Regulated by the Metastasis Suppressor BRMS1." Cancer Res 70 (24):10445-10453 (2010). DOI: 10.1158/0008-5472.CAN-10-3040. Application: Immunoprecipitation.
Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1." Biochem Biophys Res Commun 393 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. Application: Immunoblot.
Chen, G et al. "The tumor suppressor ING3 is degraded by SCFSkp2-mediated ubiquitin -proteasome system." Oncogene 29 (10):1498-1508 (2010). DOI: 10.1038/onc.2009.424. Application: Western Blot.
Chen, G et al. "The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system." Oncogene 29:1498-508 (2010). PubMed: 19935701.
Jafarnejad, SM et al. "Prognostic significance of Sox4 expression in human cutaneous melanoma and its role in cell migration and invasion." Am. J. Pathol. 177:2741-52 (2010). PubMed: 20952589. Application: Western Blot.
Lee, HS et al. "Fibroblast growth factor receptor-induced phosphorylation of ephrinB1 modulates its interaction with Dishevelled." Mol Biol Cell 20:124-33 (2009). PubMed: 19005214.
Rothenburg, S et al. "Rapid evolution of protein kinase PKR alters sensitivity to viral inhibitors." Nat. Struct. Mol. Biol. 16:63-70 (2009). PubMed: 19043413.
Prosser, DC et al. "FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation." J Cell Sci 121:3052-61 (2008). PubMed: 18713837.
Lee, HS et al. "EphrinB1 controls cell-cell junctions through the Par polarity complex." Nat. Cell Biol. 10:979-86 (2008). PubMed: 18604196.
Rothenburg, S et al. "Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: varying the number of double-stranded RNA binding domains and lineage-specific duplication." BMC Biol. 6:12 (2008). PubMed: 18312693. Application: Immunoblot.
Wang, Y et al. "Leucine zipper-like domain is required for tumor suppressor ING2-mediated nucleotide excision repair and apoptosis." FEBS Lett. 580:3787-93 (2006). PubMed: 16782091.
Cho, HJ et al. "EphrinB1 Interacts with CNK1 and Promotes Cell Migration through JNK Activation." J. Biol. Chem. : (0). PubMed: 24825906.