Rabbit anti-Ubiquitous Farnesyl Polyclonal Antibody | anti-FDPS antibody

Farnesyl

Cat. Num. AAA395838

• Gene Names: FDPS; FPS; FPPS
• Reactivity: Ubiquitous
• Applications: ELISA, Immunofluorescence
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Farnesyl; Polyclonal Antibody; anti-FDPS antibody

• Host: Rabbit
• Reactivity: Ubiquitous
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Unconjugated
• Sequence Length: 419

• Applicable Applications for anti-FDPS antibody: ELISA, Immunofluorescence (IF)
• Application Notes: Optimal concentration should be evaluated by serial dilutions.
• Immunogen: Antibody developed using farnesyl cysteine conjugated to KLH.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles; Ship Conditions: Ship at ambient temperature, freeze upon arrival.; Products are stable for one year from purchase when stored properly.

Testing Data

(Immunofluorescence assay using anti-Farnesyl antibody on plasmids encoding isoprenylated protein and visualized using FITC- conjugated goat anti-rabbit antibody )

Related Product Information for anti-FDPS antibody

Protein isoprenylation is a post-translational modification that affects about 0.5% of cellular proteins and is essential for the biological activity of proteins. Two enzymes catalyze the attachment of two prenyl groups to the sulfhydryl group of carboxyl-terminal cysteine groups. Proteins which are prenylated by these enzymes have a distinct motif at the C-terminal of the protein, C-A1-A2-X (C = Cysteine, A1 & A2 = aliphatic amino acids). The two enzymes involved in this transfer are farnesyltransferase and geranylgeranyltransferase. These transfer a 15 carbon farnesyl or a 20 carbon geranygeranyl, respectively, from a prenyl-pyrophosphate to the protein. Examples of proteins containing this C-A-A-X motif are members of the Ras small G protein family, the nuclear lamins and the gamma subunit of trimeric G proteins. Prenylation of proteins is necessary for membrane association of proteins as well as protein-protein interactions and the nature of the linked isoprenyl group can influence the protein interactions, such as the interaction between G proteins and receptors.

Product Categories/Family for anti-FDPS antibody

Polyclonal Antibody; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 209571582
• NCBI GeneID: 2224
• NCBI Accession #: NP_001129293.1
• NCBI GenBank Nucleotide #: NM_001135821.1
• UniProt Accession #: P14324; Q96G29; D3DV91; E9PCI9
• Molecular Weight: 48,275 Da
• NCBI Official Full Name: farnesyl pyrophosphate synthase isoform a
• NCBI Official Synonym Full Names: farnesyl diphosphate synthase
• NCBI Official Symbol: FDPS
• NCBI Official Synonym Symbols: FPS; FPPS
• NCBI Protein Information: farnesyl pyrophosphate synthase; FPP synthase; FPP synthetase; geranyltranstransferase; dimethylallyltranstransferase; (2E,6E)-farnesyl diphosphate synthase; farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase
• UniProt Protein Name: Farnesyl pyrophosphate synthase
• Protein Family: Farnesylcysteine lyase
• UniProt Gene Name: FDPS
• UniProt Synonym Gene Names: FPS; KIAA1293; FPP synthase; FPS
• UniProt Entry Name: FPPS_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008]

Uniprot Description

Function: Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.

Catalytic activity: Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Ref.15Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate. Ref.15

Cofactor: Binds 3 magnesium ions per subunit.

Enzyme regulation: Inactivated by interferon-induced RSAD2. This inactivation may result of disruption of lipid rafts at the plasma membrane, and thus have an antiviral effect since many enveloped viruses need lipid rafts to bud efficiently out of the cell. Ref.10

Pathway: Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.

Subunit structure: Homodimer. Interacts with RSAD2. Interacts with HTLV-1 protein p13(II). Ref.9 Ref.10 Ref.14

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the FPP/GGPP synthase family.

Sequence caution: The sequence BAA03523.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.