Mouse Cytokeratin, Basal Cell Monoclonal Antibody | anti-Krt8 antibody
Cytokeratin, Basal Cell (Basal Cell Cytokeratin)
Gene Names
Krt8; CYKER; Krt2-8
Reactivity
Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Rabbit, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
SupernatantSupernatant
Synonyms
Cytokeratin; Basal Cell; Monoclonal Antibody; Basal Cell (Basal Cell Cytokeratin); Anti -Cytokeratin; anti-Krt8 antibody
Host
Mouse
Reactivity
Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RCK
Specificity
Recognizes Basal Cell Cytokeratin. Stains basal cells in combined and stratified epithelial tissues. Species Crossreactivity: human, quail, chicken, rat, rabbit, hamster, canine, porcine, guinea pig
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Applicable Applications for anti-Krt8 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:10-1:25
Immunohistochemistry (Frozen): 1:10-1:25
Immunocytochemistry: 1:10-1:25
Dilution: Western Blot: 1:10-1:25
Immunohistochemistry (Frozen): 1:10-1:25
Immunocytochemistry: 1:10-1:25
Immunogen
Basal Cell Cytokeratin
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Krt8 antibody
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD-68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-Krt8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
54,019 Da
NCBI Official Full Name
cytokeratin 8 polypeptide
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
Krt8
NCBI Official Synonym Symbols
CYKER; Krt2-8
NCBI Protein Information
keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8
UniProt Protein Name
Keratin, type II cytoskeletal 8
Protein Family
UniProt Gene Name
Krt8
UniProt Synonym Gene Names
Krt2-8; CK-8; K8
UniProt Entry Name
K2C8_RAT
NCBI Description
an intermediate filament protein; may play a role in prostrate cells [RGD, Feb 2006]
Uniprot Description
K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.
Protein type: Cytoskeletal
Cellular Component: dystrophin-associated glycoprotein complex; nucleoplasm; intermediate filament cytoskeleton; costamere; nuclear matrix; cytoplasm; keratin filament; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma
Molecular Function: protein binding; protein complex binding; structural molecule activity
Biological Process: tumor necrosis factor-mediated signaling pathway; sarcomere organization; response to other organism; response to hydrostatic pressure
Research Articles on Krt8
Similar Products
Product Notes
The Krt8 krt8 (Catalog #AAA632277) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytokeratin, Basal Cell (Basal Cell Cytokeratin) reacts with Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytokeratin, Basal Cell can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1:10-1:25 Immunohistochemistry (Frozen): 1:10-1:25 Immunocytochemistry: 1:10-1:25. Researchers should empirically determine the suitability of the Krt8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin, Basal Cell, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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