Mouse Cytokeratin, Basal Cell Monoclonal Antibody | anti-Krt8 antibody

Cytokeratin, Basal Cell

Cat. Num. AAA609675

• Gene Names: Krt8; CYKER; Krt2-8
• Reactivity: Canine, Chicken, Hamster, Human, Porcine, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G chromatography.
• Synonyms: Cytokeratin; Basal Cell; Monoclonal Antibody; Anti -Cytokeratin; anti-Krt8 antibody

• Host: Mouse
• Reactivity: Canine, Chicken, Hamster, Human, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q1113
• Specificity: Broadly reacting cytokeratin antibody specific for cytokeratin 5. Stains basal cells in combined and stratified epithelial tissues. Recognizes the stem cell population, including the amplifying cell in the prostate epithelium. Species crossreactivity: human, rat, rabbit, hamster, canine, chicken, quail and porcine.
• Purity/Purification: Affinity Purified; Purified by Protein G chromatography.
• Form/Format: Supplied as a liquid in buffer with 0.09% sodium azide.

• Applicable Applications for anti-Krt8 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:100-1:1000; Immunohistochemistry (frozen tissue sections): 1:100-1:200
• Immunogen: Human cytokeratins.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Krt8 antibody

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.

Product Categories/Family for anti-Krt8 antibody

Antibodies; Abs to Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 203734
• NCBI GeneID: 25626
• UniProt Accession #: Q10758; Q5WPB3
• Molecular Weight: 54,019 Da
• NCBI Official Full Name: cytokeratin 8 polypeptide
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: Krt8
• NCBI Official Synonym Symbols: CYKER; Krt2-8
• NCBI Protein Information: keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: Krt8
• UniProt Synonym Gene Names: Krt2-8; CK-8; K8
• UniProt Entry Name: K2C8_RAT

NCBI Description

an intermediate filament protein; may play a role in prostrate cells [RGD, Feb 2006]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; nuclear matrix; costamere; cytoplasm; keratin filament; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; response to other organism; sarcomere organization; response to hydrostatic pressure