Mouse anti-Human Cytokeratin 19/KRT19 Monoclonal Antibody | anti-KRT19 antibody

Anti-Cytokeratin 19/KRT19 Antibody, Mouse Monoclonal

Cat. Num. AAA8100223

• Gene Names: KRT19; K19; CK19; K1CS
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: Cytokeratin 19/KRT19; Monoclonal Antibody; Anti-Cytokeratin 19/KRT19 Antibody; Mouse Monoclonal; KRT19 Antibody; Mouse MAb; keratin 19; type I; Anti-CK19 Antibody; Anti-K19 Antibody; Anti-K1CS Antibody; anti-KRT19 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG3
• Clone Number: 08
• Specificity: Human KRT19
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-KRT19 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:250-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human KRT19
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human KRT19. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human KRT19 in human thyroid cancer with mouse monoclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human KRT19 in human hepatoma with mouse monoclonal antibody (1:500, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-KRT19 antibody

Cytokeratin 19 (KRT19) is a cytoplasmic intermediate filament protein, which is responsible for structural rigidity and multipurpose scaffolds. In several cancers, KRT19 is overexpressed and may play a crucial role in tumorigenic transformation. The KRT19 gene encodes cytokeratin 19, an element of the cytoskeleton whose expression is frequently altered in renal cell carcinoma (RCC).KRT19 was identified as one of the key biomarkers for distinguishing cholangiocarcinoma (CCA) and hepatocellular carcinoma. Expression of KRT19 was specifically high in breast cancers and significantly correlated with their invasiveness. Moreover, knockdown of KRT19 led to increased proliferation, migration, invasion, drug resistance, and sphere formation in breast cancer cells via an upregulated NOTCH signaling pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 24234699
• NCBI GeneID: 3880
• NCBI Accession #: NP_002267.2
• NCBI GenBank Nucleotide #: NM_002276.4
• UniProt Accession #: P08727; Q5XG83; Q6NW33; Q7L5M9; Q96A53; Q96FV1; Q9BYF9; Q9P1Y4; B2R874
• Molecular Weight: 44,106 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 19
• NCBI Official Synonym Full Names: keratin 19
• NCBI Official Symbol: KRT19
• NCBI Official Synonym Symbols: K19; CK19; K1CS
• NCBI Protein Information: keratin, type I cytoskeletal 19; CK-19; keratin-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; 40-kDa keratin intermediate filament
• UniProt Protein Name: Keratin, type I cytoskeletal 19
• Protein Family: Keratin
• UniProt Gene Name: KRT19
• UniProt Synonym Gene Names: CK-19; K19
• UniProt Entry Name: K1C19_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: dystrophin-associated glycoprotein complex; costamere; intermediate filament; Z disc; sarcolemma

Molecular Function: protein binding; structural constituent of cytoskeleton; structural constituent of muscle; protein complex binding

Biological Process: viral reproduction; response to estrogen stimulus; sarcomere organization