Mouse anti-Human CytokeRatin 13 Monoclonal Antibody | anti-KRT13 antibody
Mouse anti CytokeRatin 13
Gene Names
KRT13; K13; CK13; MGC3781; MGC161462; KRT13
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Synonyms
CytokeRatin 13; Monoclonal Antibody; Mouse anti CytokeRatin 13; anti-KRT13 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2D7
Specificity
2D7 reacts exclusively with cytokeRatin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stRatified epithelia.
Form/Format
Each vial contains 1 ml of culture supernatant containing 0.09% sodium azide.
Applicable Applications for anti-KRT13 antibody
Immunohistochemistry (IHC) (frozen), Western Blot (WB)
Application Notes
2D7 is suitable for immunoblotting and immunohistochemistry on frozen tissues and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titRation. Recommended working dilution 1:5 -1:10.
Source Note
2D7 is a Mouse monoclonal IgG2b antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytokeRatin prepaRation extracted from Human esophagus.
Reactivity Note
Human, most mammals, including Mouse, Rat and Rabbit.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-KRT13 antibody
CytokeRatins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in Human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual Human cytokeRatins are numbered 1 to 20. The various epithelia in the Human body usually express cytokeRatins which are not only characteristic of the type of epithelium, but also related to the degree of matuRation or differentiation within an epithelium. CytokeRatin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeRatin antibodies are not only of assistance in the differential identification of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Product Categories/Family for anti-KRT13 antibody
References
van Muijen, G. N., Ruiter, D. J., Franke, W. W., Achtstatter, T., Haasnoot, W. H., Ponec, M., and Warnaar, S. O. (1986). Cell type heterogeneity of cytokeRatin expression in complex epithelia and carcinomas as demonstRated by monoclonal antibodies specific for cytokeRatins nos. 4 and 13, Exp Cell Res 162, 97-113. Weikel, W., Wagner, R., and Moll, R. (1987). Characterization of subcolumnar reserve cells and other epithelia of Human uterine cervix. DemonstRation of diverse cytokeRatin polypeptides in reserve cells, Virchows Arch B Cell Pathol Incl Mol Pathol 54, 98-110. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keRatin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. van Niekerk, C. C., Boerman, O. C., Ramaekers, F. C., and Poels, L. G. (1991). Marker profile of different phases in the transition of normal Human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455-63. Smedts, F., Ramaekers, F., Troyanovsky, S., Pruszczynski, M., Link, M., Lane, B., Leigh, I., Schijf, C., and Vooijs, P. (1992). KeRatin expression in cervical cancer, Am J Pathol 141, 497-511. Bauwens, L. J., De Groot, J. C., Ramaekers, F. C., Veldman, J. E., and Huizing, E. H. (1992). Expression of intermediate filament proteins in the adult Human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86. Van Niekerk, C. C., Ramaekers, F. C., Hanselaar, A. G., Aldeweireldt, J., and Poels, L. G. (1993). Changes in expression of differentiation markers between normal ovarian cells and derived tumors, Am J Pathol 142, 157-77. van Dorst, E. B., van Muijen, G. N., Litvinov, S. V., and Fleuren, G. J. (1998). The limited difference between keRatin patterns of squamous cell carcinomas and adenocarcinomas is explicable by both cell lineage and state of differentiation of tumour cells, J Clin Pathol 51, 679-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,588 Da
NCBI Official Full Name
cytokeratin 13
NCBI Official Synonym Full Names
keratin 13
NCBI Official Symbol
KRT13
NCBI Official Synonym Symbols
K13; CK13; MGC3781; MGC161462; KRT13
NCBI Protein Information
keratin, type I cytoskeletal 13; CK-13; keratin-13; cytokeratin 13; cytokeratin-13
UniProt Protein Name
Keratin, type I cytoskeletal 13
Protein Family
UniProt Gene Name
KRT13
UniProt Entry Name
K1C13_HUMAN
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]
Uniprot Description
K13: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K4
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: intermediate filament cytoskeleton; keratin filament; nucleus
Molecular Function: structural molecule activity
Biological Process: response to radiation; tongue morphogenesis; cytoskeleton organization and biogenesis
Disease: White Sponge Nevus 2
Research Articles on KRT13
1. Expression was altered in oral lichen planus lesions
2. Metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression.
3. The different ligand regulations of KRT13 are due to ligand-differential recruitment of ER and coactivators.
4. CK13 positive staining occurs in cervical squamous tumors and in mucinous cervical type adenocarcinomas, while there was no positive staining in non-neoplastic cervical glandular elements
2. Metaplastic squamous cells differentiate with a decrease of CK18 and an increase of CK13 expression.
3. The different ligand regulations of KRT13 are due to ligand-differential recruitment of ER and coactivators.
4. CK13 positive staining occurs in cervical squamous tumors and in mucinous cervical type adenocarcinomas, while there was no positive staining in non-neoplastic cervical glandular elements
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Product Notes
The KRT13 krt13 (Catalog #AAA570042) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CytokeRatin 13 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CytokeRatin 13 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Western Blot (WB). 2D7 is suitable for immunoblotting and immunohistochemistry on frozen tissues and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titRation. Recommended working dilution 1:5 -1:10. Researchers should empirically determine the suitability of the KRT13 krt13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CytokeRatin 13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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