Mouse anti-Human Cytochrome P450 46A1 Monoclonal Antibody | anti-CYP46A1 antibody

Cytochrome P450 46A1 (Cholesterol 24-hydroxylase, CH24H, CYP46A1, CYP46)

Cat. Num. AAA605502

• Gene Names: CYP46A1; CP46; CYP46
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Cytochrome P450 46A1; Monoclonal Antibody; Cytochrome P450 46A1 (Cholesterol 24-hydroxylase; CH24H; CYP46A1; CYP46); Anti -Cytochrome P450 46A1 (Cholesterol 24-hydroxylase; anti-CYP46A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9G345
• Specificity: Recognizes human Cytochrome P450 46A1.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, pH7.2. Reconstitute with 100ul sterile ddH2O.

• Applicable Applications for anti-CYP46A1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.
• Immunogen: Partial recombinant protein of human cytochrome P450 46A1 (aa201-300) with a GST tag.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. Aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot detection of partial recombinant human cytochrome P450 46A1 protein with GST tag, using mouse MBS605502. This antibody detects a band of molecular weight ~37KD. Note the GST tag alone has a molecular weight of 26KD.)

ELISA (EIA)

(Sandwhich ELISA using MBS605502, to capture recombinant GST tagged cytochrome P450 46A1. The detection limit for this antibody is 0.03ng/ml.)

Related Product Information for anti-CYP46A1 antibody

Cytochrome P450 46A1 belongs to the cytochrome P450 family which are a group of monooxygenases. Cytochrome P450 46A1 is involved in the turnover of cholesterol converting it into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. This protein is expressed in the brain and mRNA has been found to be broadly distributed with higher levels in the gray matter zones and lower levels in white matter areas. It is not detected in fetal brain but its expression increases linearly with age. It localises to the endoplasmic reticulum membrane and the microsome membrane. The unprocessed precursor is 500 amino acids long and has a predicted molecular weight of 56.8KD.

Product Categories/Family for anti-CYP46A1 antibody

Antibodies; Abs to Enzymes; Cytochrome

NCBI and Uniprot Product Information

• NCBI GI #: 5729796
• NCBI GeneID: 10858
• NCBI Accession #: NP_006659.1
• NCBI GenBank Nucleotide #: NM_006668.1
• UniProt Accession #: Q9Y6A2
• Molecular Weight: 56,821 Da
• NCBI Official Full Name: cholesterol 24-hydroxylase
• NCBI Official Synonym Full Names: cytochrome P450, family 46, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP46A1
• NCBI Official Synonym Symbols: CP46; CYP46
• NCBI Protein Information: cholesterol 24-hydroxylase; CH24H; cytochrome P450 46A1; cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
• UniProt Protein Name: Cholesterol 24-hydroxylase
• Protein Family: Cholesterol 24-hydroxylase
• UniProt Gene Name: CYP46A1
• UniProt Synonym Gene Names: CYP46; CH24H
• UniProt Entry Name: CP46A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP46A1: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Belongs to the cytochrome P450 family.

Protein type: Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesis; EC 1.14.13.98; Oxidoreductase

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: iron ion binding; cholesterol 24-hydroxylase activity; heme binding; steroid hydroxylase activity

Biological Process: bile acid biosynthetic process; nervous system development; bile acid metabolic process; xenobiotic metabolic process; cholesterol catabolic process; sterol metabolic process