SDS-Page
(SDS-PAGE Analysis Purified Cytochrome P450 1A1/1A2 Mouse Monoclonal Antibody (P6). Confirmation of Purity and Integrity of Antibody.)
Mouse Cytochrome P450 3A1/CYP3A1 Monoclonal Antibody | anti-CYP3A1 antibody
Cytochrome P450 3A1/CYP3A1
Gene Names
Cyp3a23/3a1; CYP; RL33; cDEX; Cyp3a1; Cyp3a3; CYP3A23
Reactivity
Human, Mouse, Rat, Pig
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Cytochrome P450 3A1/CYP3A1; Monoclonal Antibody; CYP3A1; Cytochrome P450 family 3 subfamily A polypeptide 1; anti-CYP3A1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Pig
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
P6
Specificity
Cytochrome P450 oxidase (commonly abbreviated CYP) is a generic term for a large number of related, but distinct, oxidative enzymes important in vertebrate physiology. The cytochrome P450 mixed-function monooxygenase system is probably the most important element of Phase I metabolism in mammals. P450s are membrane-bound, either in the inner membrane of mitochondria or in the endoplasmic reticulum of cells where they metabolise thousands of endogenous and exogenous compounds. In the liver, these substrates include toxins, drugs, and other unneeded and potentially harmful molecules. Humans have 18 families of cytochrome P450 genes and 43 subfamilies; the CYP1 family is involved in drug metabolism and includes 3 subfamilies, 3 genes and 1 pseudogene. The CYP2 family are part of the microsomal drug metabolising system that is responsible for oxidation of many therapeutic agents as well as steroids, fatty acids and many other endogenous substances. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. P6 is highly specific for members of the CYP 3A gene family and cross reacts with CY 3A4 in human, CYP 3A in rat and potentially CYP 3A11 (based on inducibility) in mice.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
502
Applicable Applications for anti-CYP3A1 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
ELISA: For coating, order antibody without BSA
FC/FACS: 0.5-1ug/million cells
WB: 0.5-1.0ug/ml
FC/FACS: 0.5-1ug/million cells
WB: 0.5-1.0ug/ml
Positive Control
HeLa cells. Liver or Cardiac muscle.
Immunogen
Full length protein (Rat). Rat liver cytochrome P450 3A1.
Cellular Localization
Endoplasmic reticulum, membrane bound.
Hu-chromosome Location
7q22.1
Preparation and Storage
Antibody with azide: Store at 2 to 8°C.
Antibody without azide: store at -20 to -80°C.
Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80°C.
Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis Purified Cytochrome P450 1A1/1A2 Mouse Monoclonal Antibody (P6). Confirmation of Purity and Integrity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified Cytochrome P450 1A1/1A2 Mouse Monoclonal Antibody (P6). Confirmation of Purity and Integrity of Antibody.)
References
Probert et al. 2014. Toxicol Sci. 137(2):350-70. PMID: 24235770.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51kDa
NCBI Official Full Name
cytochrome P450 3A1
NCBI Official Synonym Full Names
cytochrome P450, family 3, subfamily a, polypeptide 23/polypeptide 1
NCBI Official Symbol
Cyp3a23/3a1
NCBI Official Synonym Symbols
CYP; RL33; cDEX; Cyp3a1; Cyp3a3; CYP3A23
NCBI Protein Information
cytochrome P450 3A1
Protein Family
NCBI Description
steroid-inducible member of p450 subfamily 3A; induced by pregnenolone 16 alpha-carbonitrile, dexamethasone (DEX), phenobarbital (PB) and triacetyloleandomycin [RGD, Feb 2006]
Research Articles on CYP3A1
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Product Notes
The CYP3A1 (Catalog #AAA4381479) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 3A1/CYP3A1 reacts with Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 3A1/CYP3A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Western Blot (WB). ELISA: For coating, order antibody without BSA FC/FACS: 0.5-1ug/million cells WB: 0.5-1.0ug/ml. Researchers should empirically determine the suitability of the CYP3A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 3A1/CYP3A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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