Typical Testing Data/Standard Curve (for reference only)
Rat Cytochrome P450 3A1 ELISA Kit | Cyp3a1 elisa kit
Rat Cytochrome P450 3A1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 2E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cytochrome P450 2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
steroid-inducible member of p450 subfamily 3A; induced by pregnenolone 16 alpha-carbonitrile, dexamethasone (DEX), phenobarbital (PB) and triacetyloleandomycin [RGD, Feb 2006]
Uniprot Description
CYP3A5: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Lipid Metabolism - linoleic acid; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - drug metabolism - other enzymes; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 12p11
Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle
Molecular Function: demethylase activity; testosterone 6-beta-hydroxylase activity
Biological Process: aging; drug metabolic process; response to cadmium ion; response to drug; response to glucocorticoid stimulus; response to metal ion; response to nutrient
Research Articles on Cyp3a1
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Product Notes
The Rat Cyp3a1 cyp3a1 (Catalog #AAA9428186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9428186 ELISA Kit recognizes Rat Cyp3a1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 3A1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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