Mouse anti-Human CD79a Monoclonal Antibody | anti-CD79A antibody

CD79a (B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA4380127

• Gene Names: CD79A; IGA; MB-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CD79a; Monoclonal Antibody; CD79a (B-Cell Marker) Mouse Monoclonal Antibody; B lymphocyte-specific MB1 protein; B-cell antigen receptor complex-associated protein alpha chain; CD79a molecule immunoglobulin associated alpha; Ig-alpha; IGA; IgM-alpha; Immunoglobulin-associated alpha; Ly54; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein; anti-CD79A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: JCB117
• Specificity: A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
• Form/Format: Bioreactor Concentrate with 0.05% Azide.
• Sequence Length: 226

• Applicable Applications for anti-CD79A antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (5-10ul/million cells in 0.1ml); Immunofluorescence (1:50-1:100); Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cell Surface
• Immunogen: A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein.
• Hu-Chromosome Location: 19q13.2
• Positive Control: Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.
• Preparation and Storage: Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD79a Ab (JCB117).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD79a Ab (JCB117).)

References

Mason, DY, et al. 1995. Blood 86: 1453-1459

NCBI and Uniprot Product Information

• NCBI GI #: 4502685
• NCBI GeneID: 973
• NCBI Accession #: NP_001774.1
• NCBI GenBank Nucleotide #: NM_001783.3
• UniProt Accession #: P11912; Q53FB8; A0N775
• Molecular Weight: 44kDa
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein alpha chain isoform 1
• NCBI Official Synonym Full Names: CD79a molecule
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: B cell receptor complex; cytoplasm; external side of plasma membrane; integral to plasma membrane; lipid raft; multivesicular body; plasma membrane

Molecular Function: protein binding

Biological Process: B cell activation; B cell differentiation; B cell proliferation; B cell receptor signaling pathway

Disease: Agammaglobulinemia 3, Autosomal Recessive